High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy

Department of Drug Design and Pharmacology

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. / Gromova, Irina; Gromov, Pavel; Honma, Naoko; Kumar, Sudha; Rimm, David; Talman, Maj-Lis Møller; Wielenga, Vera Timmermans; Moreira, José.

In: Molecular Oncology, Vol. 9, No. 8, 2015, p. 1636-1654.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gromova, I, Gromov, P, Honma, N, Kumar, S, Rimm, D, Talman, M-LM, Wielenga, VT & Moreira, J 2015, 'High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy', Molecular Oncology, vol. 9, no. 8, pp. 1636-1654. https://doi.org/10.1016/j.molonc.2015.05.003

APA

Gromova, I., Gromov, P., Honma, N., Kumar, S., Rimm, D., Talman, M-L. M., Wielenga, V. T., & Moreira, J. (2015). High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. Molecular Oncology, 9(8), 1636-1654. https://doi.org/10.1016/j.molonc.2015.05.003

Vancouver

Gromova I, Gromov P, Honma N, Kumar S, Rimm D, Talman M-LM et al. High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. Molecular Oncology. 2015;9(8):1636-1654. https://doi.org/10.1016/j.molonc.2015.05.003

Author

Gromova, Irina ; Gromov, Pavel ; Honma, Naoko ; Kumar, Sudha ; Rimm, David ; Talman, Maj-Lis Møller ; Wielenga, Vera Timmermans ; Moreira, José. / High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. In: Molecular Oncology. 2015 ; Vol. 9, No. 8. pp. 1636-1654.

Bibtex

@article{0f1b528d58be42c78f2ca648355d195e,

title = "High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy",

abstract = "We have previously reported the 2D PAGE-based proteomic profiling of a prospective cohort of 78 triple negative breast cancer (TNBC) patients, and the establishment of a cumulative TNBC protein database. Analysis of this database identified a number of proteins as being specifically overexpressed in TNBC samples. One such protein was D-3-phosphoglycerate dehydrogenase (Phgdh), a candidate oncogene. We analysed expression of Phgdh in normal and TNBC mammary tissue samples by 2D gel-based proteomics and immunohistochemistry (IHC), and show here that high-level expression of Phgdh in mammary epithelial cells is primarily associated with cell lineage, as we found that Phgdh expression was predominant in CK5-positive cells, normal as well as malignant, thus identifying an association of this protein with the basal phenotype. Quantitative IHC analysis of Phgdh expression in normal breast tissue showed high-level expression of Phgdh in normal CK5-positive mammary epithelial cells, indicating that expression of this protein was not associated with malignancy, but rather with cell lineage. However, proteomic profiling of Phgdh showed it to be expressed in two major protein forms, and that the ratio of expression between these variants was associated with malignancy. Overexpression of Phgdh in CK5-positive cell lineages, and differential protein isoform expression, was additionally found in other tissues and cancer types, suggesting that overexpression of Phgdh is generally associated with CK5 cells, and that oncogenic function may be determined by isoform expression.",

author = "Irina Gromova and Pavel Gromov and Naoko Honma and Sudha Kumar and David Rimm and Talman, {Maj-Lis M{\o}ller} and Wielenga, {Vera Timmermans} and Jos{\'e} Moreira",

year = "2015",

doi = "10.1016/j.molonc.2015.05.003",

language = "English",

volume = "9",

pages = "1636--1654",

journal = "Molecular Oncology",

issn = "1574-7891",

publisher = "Elsevier",

number = "8",

}

RIS

TY - JOUR

T1 - High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy

AU - Gromova, Irina

AU - Gromov, Pavel

AU - Honma, Naoko

AU - Kumar, Sudha

AU - Rimm, David

AU - Talman, Maj-Lis Møller

AU - Wielenga, Vera Timmermans

AU - Moreira, José

PY - 2015

Y1 - 2015

N2 - We have previously reported the 2D PAGE-based proteomic profiling of a prospective cohort of 78 triple negative breast cancer (TNBC) patients, and the establishment of a cumulative TNBC protein database. Analysis of this database identified a number of proteins as being specifically overexpressed in TNBC samples. One such protein was D-3-phosphoglycerate dehydrogenase (Phgdh), a candidate oncogene. We analysed expression of Phgdh in normal and TNBC mammary tissue samples by 2D gel-based proteomics and immunohistochemistry (IHC), and show here that high-level expression of Phgdh in mammary epithelial cells is primarily associated with cell lineage, as we found that Phgdh expression was predominant in CK5-positive cells, normal as well as malignant, thus identifying an association of this protein with the basal phenotype. Quantitative IHC analysis of Phgdh expression in normal breast tissue showed high-level expression of Phgdh in normal CK5-positive mammary epithelial cells, indicating that expression of this protein was not associated with malignancy, but rather with cell lineage. However, proteomic profiling of Phgdh showed it to be expressed in two major protein forms, and that the ratio of expression between these variants was associated with malignancy. Overexpression of Phgdh in CK5-positive cell lineages, and differential protein isoform expression, was additionally found in other tissues and cancer types, suggesting that overexpression of Phgdh is generally associated with CK5 cells, and that oncogenic function may be determined by isoform expression.

AB - We have previously reported the 2D PAGE-based proteomic profiling of a prospective cohort of 78 triple negative breast cancer (TNBC) patients, and the establishment of a cumulative TNBC protein database. Analysis of this database identified a number of proteins as being specifically overexpressed in TNBC samples. One such protein was D-3-phosphoglycerate dehydrogenase (Phgdh), a candidate oncogene. We analysed expression of Phgdh in normal and TNBC mammary tissue samples by 2D gel-based proteomics and immunohistochemistry (IHC), and show here that high-level expression of Phgdh in mammary epithelial cells is primarily associated with cell lineage, as we found that Phgdh expression was predominant in CK5-positive cells, normal as well as malignant, thus identifying an association of this protein with the basal phenotype. Quantitative IHC analysis of Phgdh expression in normal breast tissue showed high-level expression of Phgdh in normal CK5-positive mammary epithelial cells, indicating that expression of this protein was not associated with malignancy, but rather with cell lineage. However, proteomic profiling of Phgdh showed it to be expressed in two major protein forms, and that the ratio of expression between these variants was associated with malignancy. Overexpression of Phgdh in CK5-positive cell lineages, and differential protein isoform expression, was additionally found in other tissues and cancer types, suggesting that overexpression of Phgdh is generally associated with CK5 cells, and that oncogenic function may be determined by isoform expression.

U2 - 10.1016/j.molonc.2015.05.003

DO - 10.1016/j.molonc.2015.05.003

M3 - Journal article

C2 - 26026368

VL - 9

SP - 1636

EP - 1654

JO - Molecular Oncology

JF - Molecular Oncology

SN - 1574-7891

IS - 8

ER -

ID: 145476625