Metallopeptidase Inhibitor 1 (TIMP-1) promotes receptor tyrosine kinase c-Kit signaling in colorectal cancer
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Metallopeptidase Inhibitor 1 (TIMP-1) promotes receptor tyrosine kinase c-Kit signaling in colorectal cancer. / Nordgaard, Cathrine; Doll, Sophia; Matos, Ana Laura de Souza Almeida; Høeberg, Mikkel; Kazi, Julhash Uddin; Friis, Stine; Stenvang, Jan; Rönnstrand, Lars; Mann, Matthias; Moreira, José Manuel Afonso.
In: Molecular Oncology, Vol. 13, No. 12, 2019, p. 2646-2662.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Metallopeptidase Inhibitor 1 (TIMP-1) promotes receptor tyrosine kinase c-Kit signaling in colorectal cancer
AU - Nordgaard, Cathrine
AU - Doll, Sophia
AU - Matos, Ana Laura de Souza Almeida
AU - Høeberg, Mikkel
AU - Kazi, Julhash Uddin
AU - Friis, Stine
AU - Stenvang, Jan
AU - Rönnstrand, Lars
AU - Mann, Matthias
AU - Moreira, José Manuel Afonso
PY - 2019
Y1 - 2019
N2 - Colorectal cancer (CRC) is the third most prevalent cancer worldwide causing an estimated 700000 deaths annually. Different types of treatment are available for patients with advanced metastatic colorectal cancer (mCRC), including targeted biological agents, such as cetuximab, a monoclonal antibody that targets EGFR. We have previously reported a study indicating multiple levels of interaction between Metallopeptidase Inhibitor 1 (TIMP)-1 and the epidermal growth factor (EGF) signaling axis, which could explain how TIMP-1 levels can affect the anti-tumor effects of EGFR inhibitors. We also reported an association between TIMP-1-mediated cell invasive behavior and KRAS status.To gain insight into the molecular mechanisms underlying the effects of TIMP-1 in CRC, we examined by transcriptomics, proteomics and kinase-activity profiling a matched pair of isogenic human CRC isogenic DLD-1 CRC cell clones, bearing either an hemizygous KRAS wild-type allele or KRAS G13D mutant allele, and exposed, or not, to TIMP-1. Omics analysis of the two cell lines identified the receptor tyrosine kinase c-Kit, a proto-oncogene that can modulate cell proliferation and invasion in CRC, as a target for TIMP-1. We found that exposure of DLD-1 CRC cells to exogenously added TIMP-1 promoted phosphorylation of c-Kit, indicative of a stimulatory effect of TIMP-1 on the c-Kit signaling axis. In addition, TIMP-1 inhibited c-Kit shedding in CRC cells grown in the presence of exogenous TIMP-1. Given the regulatory roles that c-Kit plays in cell proliferation and migration, and the realization that c-Kit is an important oncogene in CRC, it is likely that some of the biological effects of TIMP-1 overexpression in CRC may be exerted through its effect on c-Kit signaling.
AB - Colorectal cancer (CRC) is the third most prevalent cancer worldwide causing an estimated 700000 deaths annually. Different types of treatment are available for patients with advanced metastatic colorectal cancer (mCRC), including targeted biological agents, such as cetuximab, a monoclonal antibody that targets EGFR. We have previously reported a study indicating multiple levels of interaction between Metallopeptidase Inhibitor 1 (TIMP)-1 and the epidermal growth factor (EGF) signaling axis, which could explain how TIMP-1 levels can affect the anti-tumor effects of EGFR inhibitors. We also reported an association between TIMP-1-mediated cell invasive behavior and KRAS status.To gain insight into the molecular mechanisms underlying the effects of TIMP-1 in CRC, we examined by transcriptomics, proteomics and kinase-activity profiling a matched pair of isogenic human CRC isogenic DLD-1 CRC cell clones, bearing either an hemizygous KRAS wild-type allele or KRAS G13D mutant allele, and exposed, or not, to TIMP-1. Omics analysis of the two cell lines identified the receptor tyrosine kinase c-Kit, a proto-oncogene that can modulate cell proliferation and invasion in CRC, as a target for TIMP-1. We found that exposure of DLD-1 CRC cells to exogenously added TIMP-1 promoted phosphorylation of c-Kit, indicative of a stimulatory effect of TIMP-1 on the c-Kit signaling axis. In addition, TIMP-1 inhibited c-Kit shedding in CRC cells grown in the presence of exogenous TIMP-1. Given the regulatory roles that c-Kit plays in cell proliferation and migration, and the realization that c-Kit is an important oncogene in CRC, it is likely that some of the biological effects of TIMP-1 overexpression in CRC may be exerted through its effect on c-Kit signaling.
U2 - 10.1002/1878-0261.12575
DO - 10.1002/1878-0261.12575
M3 - Journal article
C2 - 31545548
VL - 13
SP - 2646
EP - 2662
JO - Molecular Oncology
JF - Molecular Oncology
SN - 1574-7891
IS - 12
ER -
ID: 228085635