Proximity probing assays for simultaneous visualization of protein complexes in situ

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Proximity probing assays for simultaneous visualization of protein complexes in situ. / Moreira, José; Thorsen, Stine Buch; Brünner, Nils; Stenvang, Jan.

In: Expert Review of Proteomics, Vol. 10, No. 3, 2013, p. 219-221.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Moreira, J, Thorsen, SB, Brünner, N & Stenvang, J 2013, 'Proximity probing assays for simultaneous visualization of protein complexes in situ', Expert Review of Proteomics, vol. 10, no. 3, pp. 219-221. https://doi.org/10.1586/epr.13.22

APA

Moreira, J., Thorsen, S. B., Brünner, N., & Stenvang, J. (2013). Proximity probing assays for simultaneous visualization of protein complexes in situ. Expert Review of Proteomics, 10(3), 219-221. https://doi.org/10.1586/epr.13.22

Vancouver

Moreira J, Thorsen SB, Brünner N, Stenvang J. Proximity probing assays for simultaneous visualization of protein complexes in situ. Expert Review of Proteomics. 2013;10(3):219-221. https://doi.org/10.1586/epr.13.22

Author

Moreira, José ; Thorsen, Stine Buch ; Brünner, Nils ; Stenvang, Jan. / Proximity probing assays for simultaneous visualization of protein complexes in situ. In: Expert Review of Proteomics. 2013 ; Vol. 10, No. 3. pp. 219-221.

Bibtex

@article{504b10cb8b604f45b2df94cbf27054c9,
title = "Proximity probing assays for simultaneous visualization of protein complexes in situ",
abstract = "EVALUATION OF: Leuchowius KJ, Clausson CM, Grannas K et al. Parallel visualization of multiple protein complexes in individual cells in tumor tissue. Mol. Cell Proteomics doi:10.1074/mcp.O112.023374 (2013) (Epub ahead of print). Techniques for in situ detection and quantification of proteins in fixed tissue remain an important element of both basic biological analyses and clinical biomarker research. The practical importance of such techniques can be exemplified by the everyday clinical use of immunohistochemical detection of the estrogen receptor and HER2 in tissues from breast cancer patients. Several techniques are currently available for detection of single proteins and post-translational modifications, but very few are suitable for detection of protein complexes. Methods that enable simultaneous detection and quantification of protein complexes provide novel possibilities for understanding the biological role(s) of protein complexes and may open new opportunities to improve clinical biomarker research. Leuchowius et al. describe an improved proximity ligation assay for in situ detection of protein complexes, which is able to detect and quantify several protein complexes simultaneously in the same tissue specimen.",
author = "Jos{\'e} Moreira and Thorsen, {Stine Buch} and Nils Br{\"u}nner and Jan Stenvang",
year = "2013",
doi = "10.1586/epr.13.22",
language = "English",
volume = "10",
pages = "219--221",
journal = "Expert Review of Proteomics",
issn = "1478-9450",
publisher = "Taylor & Francis",
number = "3",

}

RIS

TY - JOUR

T1 - Proximity probing assays for simultaneous visualization of protein complexes in situ

AU - Moreira, José

AU - Thorsen, Stine Buch

AU - Brünner, Nils

AU - Stenvang, Jan

PY - 2013

Y1 - 2013

N2 - EVALUATION OF: Leuchowius KJ, Clausson CM, Grannas K et al. Parallel visualization of multiple protein complexes in individual cells in tumor tissue. Mol. Cell Proteomics doi:10.1074/mcp.O112.023374 (2013) (Epub ahead of print). Techniques for in situ detection and quantification of proteins in fixed tissue remain an important element of both basic biological analyses and clinical biomarker research. The practical importance of such techniques can be exemplified by the everyday clinical use of immunohistochemical detection of the estrogen receptor and HER2 in tissues from breast cancer patients. Several techniques are currently available for detection of single proteins and post-translational modifications, but very few are suitable for detection of protein complexes. Methods that enable simultaneous detection and quantification of protein complexes provide novel possibilities for understanding the biological role(s) of protein complexes and may open new opportunities to improve clinical biomarker research. Leuchowius et al. describe an improved proximity ligation assay for in situ detection of protein complexes, which is able to detect and quantify several protein complexes simultaneously in the same tissue specimen.

AB - EVALUATION OF: Leuchowius KJ, Clausson CM, Grannas K et al. Parallel visualization of multiple protein complexes in individual cells in tumor tissue. Mol. Cell Proteomics doi:10.1074/mcp.O112.023374 (2013) (Epub ahead of print). Techniques for in situ detection and quantification of proteins in fixed tissue remain an important element of both basic biological analyses and clinical biomarker research. The practical importance of such techniques can be exemplified by the everyday clinical use of immunohistochemical detection of the estrogen receptor and HER2 in tissues from breast cancer patients. Several techniques are currently available for detection of single proteins and post-translational modifications, but very few are suitable for detection of protein complexes. Methods that enable simultaneous detection and quantification of protein complexes provide novel possibilities for understanding the biological role(s) of protein complexes and may open new opportunities to improve clinical biomarker research. Leuchowius et al. describe an improved proximity ligation assay for in situ detection of protein complexes, which is able to detect and quantify several protein complexes simultaneously in the same tissue specimen.

U2 - 10.1586/epr.13.22

DO - 10.1586/epr.13.22

M3 - Journal article

C2 - 23777212

VL - 10

SP - 219

EP - 221

JO - Expert Review of Proteomics

JF - Expert Review of Proteomics

SN - 1478-9450

IS - 3

ER -

ID: 59311710