Laura Mikél McNair
Guest Researcher, Assistant Professor
Neuropharm and Genetics
Blegdamsvej 3
2200 København N.
Molecular and Cellular Pharmacology
Jagtvej 160
2100 København Ø
Member of:
Introductory remarks on publicationslist
My latest (unpublished) results comprise the identification of compounds isolated from Eremophila species, capable of both potentiating and inhibiting the transport of dopamine through the dopamine transporter (DAT). I am currently seeking to characterise these compounds further and elucidate the mechanism by which they exert their action.
During my PostDoc with Professor Dan Stærk (2016-2019), conducted in tight collaboration with a food company, we identified and characterized a new antifungal peptide in fermented milk product containing bioprotective Lactobacillus cultures (McNair et al. 2018 FEMS Yeast), and established that competitive exclusion is a major bioprotective mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products (Siedler et al. 2020 Appl Environ Microbiol).
Supervision of students during my PostDoc allowed me to contribute to excellent natural product research by Dr. Abd Malik, identifying novel antidiabetic zanthones in extracts of an endophyte (Malik et al. 2020 Fitoterapia), and by Maliheh Najari Beidokhti, highlighting the antidiabetic potential of ethanolic extracts of P. guajava leaves using multiple assays (Beidokhti et al. 2020 J Ethnopharmacol).
The work from my PhD (2013-2016) include thorough characterisation of cellular energy metabolism in acutely isolated hippocampal and cerebral cortical mouse brain slices using 13C-mapping, GC-MS and HPLC (McNair et al. 2017 Neurochem Res) and provides evidence of metabolic similarity of these two brain regions. Moreover, I propose new interesting approaches for interpreting 13C-mapping data. This work was followed-up by evaluating the specificity of 13C-substrates for assessing astrocytic and neuronal metabolism individually in acute brain slices (Andersen, McNair et al. 2017 J Neurosci Res). Other parts of my work in acute brain slices have contributed to a recent review discussing the role of glutamate dehydrogenase and amino transferases in connection to glutamate oxidation in astrocytes (McKenna, Stridt, McNair et al. 2016 J Neurosci Res).
As part of my PhD I also completed two large rodent ex vivo studies combining 13C-mapping with GC-MS and 13C/1H NMR, in collaboration with excellent researchers at Yale University and Harvard Medical School (USA), respectively. I reveal significant brain regional differences in the rate of pyruvate carboxylase, an astrocytic enzyme essential for de novo synthesis of the excitatory neurotransmitter glutamate (manuscript in progress). Furthermore, I provide data suggesting that the glutamate transporter GLT-1 expressed in neurons is important for glutamate homeostasis and synaptic energy metabolism in cerebral cortex (McNair et al. 2019 J Neurosci). Additional studies extend this hypothesis to the hippocampus and striatum (McNair et al. 2020 NERE)
Results from my undergraduate research (2011-2012) at Bath University (UK) provide indications of differential changes in neural plasticity induced by addictive drug exposure with and without the context of a place-preference learning task, measured as long-term potentiation in hippocampal slices. This work was presented on scientific poster at the British Pharmacology Society meeting in 2012 and my data formed the basis for further work within Prof. C. Bailey's group.
Finally, my Master’s thesis elucidates the significant impact of pre-natal exposure to nicotine on brain function, measured as calcium responses to glutamatergic stimulation in brain slice from pubs born of nicotine treated mice (McNair & Kohlmeier 2015 J Dev Orig Health Dis).
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Glutamate homeostasis revisited - neuronal transport and metabolism
Waagepetersen, Helle S., McNair, Laura M., Christensen, S. K., Andersen, Jens Velde & Aldana, Blanca, 2019, p. 19-19.Research output: Contribution to conference › Conference abstract for conference › Research › peer-review
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Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products
Siedler, S., Rau, M. H., Bidstrup, S., Vento, J. M., Aunsbjerg, S. D., Bosma, E. F., McNair, Laura M., Beisel, C. L. & Neves, A. R., 2020, In: Applied and Environmental Microbiology. 86, 7, 14 p., ARTN e02312-19.Research output: Contribution to journal › Journal article › Research › peer-review
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Biodiscoveries within the Australian plant genus Eremophila based on international and interdisciplinary collaboration: results and perspectives on outstanding ethical dilemmas
Semple, S., Stærk, Dan, Buirchell, B., Fowler, R., Gericke, Oliver, Kjaerulff, Louise, Zhao, Yong, Pedersen, H. A., Petersen, M. J., Rasmussen, L. F., Bredahl, E. K., Pedersen, Gustav Blichfeldt, McNair, Laura M., Ndi, C., Hansen, N. L., Heskes, A., Bayly, M., Loland, Claus Juul, Heinz, Nanna & Møller, Birger Lindberg, 2022, In: Plant Journal. 111, 4, p. 936-953 15866.Research output: Contribution to journal › Journal article › Research › peer-review
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Stable isotope tracing reveals disturbed cellular energy and glutamate metabolism in hippocampal slices of aged male mice
McNair, Laura M., Andersen, Jens Velde & Waagepetersen, Helle S., 2023, In: Neurochemistry International. 171, 10 p., 105626.Research output: Contribution to journal › Journal article › Research › peer-review
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Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo
McNair, Laura M., Mason, G. F., Chowdhury, G. M. I., Jiang, L., Ma, X., Rothman, D. L., Waagepetersen, Helle S. & Behar, K. L., 2022, In: Journal of Cerebral Blood Flow and Metabolism. 42, 8, p. 1507–1523Research output: Contribution to journal › Journal article › Research › peer-review
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Identification and characterization of a new antifungal peptide in fermented milk product containing bioprotective Lactobacillus cultures
McNair, Laura M., Siedler, S., Vinther, J. M., Hansen, A. M., Neves, A. R., Jäger, A. K., Franzyk, Henrik & Stærk, Dan, 2018, In: F E M S Yeast Research. 18, 9 p., foy094.Research output: Contribution to journal › Journal article › Research › peer-review
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Deletion of neuronal GLT-1 in mice reveals its role in synaptic glutamate homeostasis and mitochondrial function
McNair, Laura M., Andersen, Jens Velde, Aldana, Blanca, Hohnholt, M. C., Nissen, J. D., Sun, Y., Fischer, K. D., Sonnewald, U., Nyberg, N., Webster, S. C., Kapur, K., Rimmele, T. S., Barone, I., Hawks-Mayer, H., Lipton, J. O., Hodgson, N. W., Aoki, C. J., Rosenberg, P. A. & Waagepetersen, Helle S., 2019, In: The Journal of neuroscience : the official journal of the Society for Neuroscience. 39, 25, p. 4847-4863Research output: Contribution to journal › Journal article › Research › peer-review
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Conditional Knockout of GLT-1 in Neurons Leads to Alterations in Aspartate Homeostasis and Synaptic Mitochondrial Metabolism in Striatum and Hippocampus
McNair, Laura M., Andersen, Jens Velde, Nissen, J. D., Sun, Y., Fischer, K. D., Hodgson, N. W., Du, M., Aoki, C. J., Waagepetersen, Helle S., Rosenberg, P. A. & Aldana, Blanca, 2020, In: Neurochemical Research. 45, p. 1420–1437 18 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Metabolic Characterization of Acutely Isolated Hippocampal and Cerebral Cortical Slices Using [U-(13)C]Glucose and [1,2-(13)C]Acetate as Substrates
McNair, Laura M., Kornfelt, R., Walls, Anne Byriel, Andersen, Jens Velde, Aldana, B. I., Nissen, J. D., Schousboe, Arne & Waagepetersen, Helle S., 2017, In: Neurochemical Research. 42, 3, p. 810-826 17 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Glutamate oxidation in astrocytes: Roles of glutamate dehydrogenase and aminotransferases
McKenna, M. C., Stridh, M. H., McNair, Laura M., Sonnewald, U., Waagepetersen, Helle S. & Schousboe, Arne, 15 Sep 2016, In: Journal of Neuroscience Research. 94, 12, p. 1561-1571 11 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Prenatal nicotine is associated with reduced AMPA and NMDA receptor-mediated rises in calcium within the laterodorsal tegmentum: a pontine nucleus involved in addiction processes
McNair, Laura M. & Kohlmeier, Kristi Anne, 2015, In: Journal of Developmental Origins of Health and Disease. 6, 3, p. 225-41 17 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Antidiabetic xanthones with α-glucosidase inhibitory activities from an endophytic Penicillium canescens
Malik, A., Ardalani, H., Anam, S., McNair, Laura M., Kromphardt, K. J. K., Frandsen, R. J. N., Franzyk, Henrik, Stærk, Dan & Kongstad, Kenneth Thermann, 2020, In: Fitoterapia. 142, 8 p., 104522.Research output: Contribution to journal › Journal article › Research › peer-review
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Evaluation of the antidiabetic potential of Psidium guajava L. (Myrtaceae) using assays for α-glucosidase, α-amylase, muscle glucose uptake, liver glucose production and triglyceride accumulation in adipocytes
Beidokhti, M. N., Eid, H. M., Villavicencio, M. L. S., Jäger, A. K., Lobbens, E. S., Rasoanaivo, P. R., McNair, Laura M., Haddad, P. S. & Stærk, Dan, 17 Apr 2020, In: Journal of Ethnopharmacology. 257, 8 p., 112877.Research output: Contribution to journal › Journal article › Research › peer-review
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Specificity of exogenous acetate and glutamate as astrocyte substrates examined in acute brain slices from female mice using methionine sulfoximine (MSO) to inhibit glutamine synthesis
Andersen, Jens Velde, McNair, Laura M., Schousboe, Arne & Waagepetersen, Helle S., 2017, In: Journal of Neuroscience Research. 95, 11, p. 2207-2216Research output: Contribution to journal › Journal article › Research › peer-review
ID: 44491929
Most downloads
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169
downloads
Glutamate homeostasis revisited - neuronal transport and metabolism
Research output: Contribution to conference › Conference abstract for conference › Research › peer-review
Published -
23
downloads
Biodiscoveries within the Australian plant genus Eremophila based on international and interdisciplinary collaboration: results and perspectives on outstanding ethical dilemmas
Research output: Contribution to journal › Journal article › Research › peer-review
Published -
10
downloads
Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products
Research output: Contribution to journal › Journal article › Research › peer-review
Published