Chronic administration of hydrolysed pine nut oil to mice improves insulin sensitivity and glucose tolerance and increases energy expenditure via a free fatty acid receptor 4-dependent mechanism
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Chronic administration of hydrolysed pine nut oil to mice improves insulin sensitivity and glucose tolerance and increases energy expenditure via a free fatty acid receptor 4-dependent mechanism. / Wargent, Edward T.; Kȩpczyńska, Małgorzata A.; Kaspersen, Mads H.; Ulven, Elisabeth Rexen; Arch, Jonathan R.S.; Ulven, Trond; Stocker, Claire J.
In: British Journal of Nutrition, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Chronic administration of hydrolysed pine nut oil to mice improves insulin sensitivity and glucose tolerance and increases energy expenditure via a free fatty acid receptor 4-dependent mechanism
AU - Wargent, Edward T.
AU - Kȩpczyńska, Małgorzata A.
AU - Kaspersen, Mads H.
AU - Ulven, Elisabeth Rexen
AU - Arch, Jonathan R.S.
AU - Ulven, Trond
AU - Stocker, Claire J.
N1 - Publisher Copyright: © The Authors 2024.
PY - 2024
Y1 - 2024
N2 - A healthy diet is at the forefront of measures to prevent type 2 diabetes. Certain vegetable and fish oils, such as pine nut oil (PNO), have been demonstrated to ameliorate the adverse metabolic effects of a high-fat diet. The present study study investigates the involvement of the free fatty acid receptors 1 (FFAR1) and 4 (FFAR4) in the chronic activity of hydrolysed PNO (hPNO) on high-fat diet-induced obesity and insulin resistance. Male C57BL/6J wild-type, FFAR1 knockout (-/-) and FFAR4-/- mice were placed on 60% high-fat diet for 3 months. Mice were then dosed hPNO for 24 days, during which time body composition, energy intake and expenditure, glucose tolerance and fasting plasma insulin, leptin and adiponectin were measured. hPNO improved glucose tolerance and decreased plasma insulin in the wild-type and FFAR1-/- mice, but not the FFAR4-/- mice. hPNO also decreased high-fat diet-induced bodyweight gain and fat mass, whilst increasing energy expenditure and plasma adiponectin. None of these effects on energy balance were statistically significant in FFAR4-/- mice but it was not shown that they were significantly less than in wild-type mice. In conclusion, chronic hPNO supplementation reduces the metabolically detrimental effects of high-fat diet on obesity and insulin resistance in a manner that is dependent on the presence of FFAR4.
AB - A healthy diet is at the forefront of measures to prevent type 2 diabetes. Certain vegetable and fish oils, such as pine nut oil (PNO), have been demonstrated to ameliorate the adverse metabolic effects of a high-fat diet. The present study study investigates the involvement of the free fatty acid receptors 1 (FFAR1) and 4 (FFAR4) in the chronic activity of hydrolysed PNO (hPNO) on high-fat diet-induced obesity and insulin resistance. Male C57BL/6J wild-type, FFAR1 knockout (-/-) and FFAR4-/- mice were placed on 60% high-fat diet for 3 months. Mice were then dosed hPNO for 24 days, during which time body composition, energy intake and expenditure, glucose tolerance and fasting plasma insulin, leptin and adiponectin were measured. hPNO improved glucose tolerance and decreased plasma insulin in the wild-type and FFAR1-/- mice, but not the FFAR4-/- mice. hPNO also decreased high-fat diet-induced bodyweight gain and fat mass, whilst increasing energy expenditure and plasma adiponectin. None of these effects on energy balance were statistically significant in FFAR4-/- mice but it was not shown that they were significantly less than in wild-type mice. In conclusion, chronic hPNO supplementation reduces the metabolically detrimental effects of high-fat diet on obesity and insulin resistance in a manner that is dependent on the presence of FFAR4.
KW - energy expenditure
KW - FFAR1
KW - FFAR4
KW - glucose tolerance
KW - high-fat diet
KW - insulin resistance
KW - pine nut oil
U2 - 10.1017/S0007114524000965
DO - 10.1017/S0007114524000965
M3 - Journal article
C2 - 38751244
AN - SCOPUS:85193784170
JO - British Journal of Nutrition
JF - British Journal of Nutrition
SN - 0007-1145
ER -
ID: 393774905