Effect of Ageing on the Passive and Active Tension and Pharmacodynamic Characteristics of Rat Coronary Arteries: Age-Dependent Increase in Sensitivity to 5-HT and K+
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Effect of Ageing on the Passive and Active Tension and Pharmacodynamic Characteristics of Rat Coronary Arteries : Age-Dependent Increase in Sensitivity to 5-HT and K+. / Sheykhzade, Majid; Simonsen, Anja Hviid ; Boonen, Harrie C.M.; Outzen, Emilie Middelbo; Nyborg, Niels Chresten Berg.
In: Pharmacology, Vol. 90, No. 3-4, 22.08.2012, p. 160-168.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of Ageing on the Passive and Active Tension and Pharmacodynamic Characteristics of Rat Coronary Arteries
T2 - Age-Dependent Increase in Sensitivity to 5-HT and K+
AU - Sheykhzade, Majid
AU - Simonsen, Anja Hviid
AU - Boonen, Harrie C.M.
AU - Outzen, Emilie Middelbo
AU - Nyborg, Niels Chresten Berg
PY - 2012/8/22
Y1 - 2012/8/22
N2 - The influence of ageing on the passive and active tension and pharmacodynamic characteristics of intramural coronary arteries from 3-month-old and 2-year-old male Wistar rats was investigated using an isometric myograph. The passive vessel wall tension measured in Ca2+-free physiological salt solution at L0 was significantly greater in arteries from old rats (1.46 ± 0.10 Nm–1, n = 7) than in young rats (1.13 ± 0.13 Nm–1, n = 6). However, the maximal active tension at L0 was similar. The spontaneous myogenic tone was increased by age and the vasorelaxation induced by extracellular K+ was significantly higher in coronary arteries of old rats. The sensitivity (pD2) to 5-HT was significantly higher in arteries from old (6.43 ± 0.11, n = 22) than from young rats (6.16 ± 0.08, n = 29). Ketanserin induced a concentration-dependent rightward shift of the 5-HT concentration-response curve in arteries from both young and old rats. The slopes of the regression lines of the Schild plots were not significantly different from unity and the estimated pKB values for ketanserin were similar. In conclusion, ageing is associated with changes in passive mechanical characteristics as well as changes in pharmacological properties in rat coronary small arteries.
AB - The influence of ageing on the passive and active tension and pharmacodynamic characteristics of intramural coronary arteries from 3-month-old and 2-year-old male Wistar rats was investigated using an isometric myograph. The passive vessel wall tension measured in Ca2+-free physiological salt solution at L0 was significantly greater in arteries from old rats (1.46 ± 0.10 Nm–1, n = 7) than in young rats (1.13 ± 0.13 Nm–1, n = 6). However, the maximal active tension at L0 was similar. The spontaneous myogenic tone was increased by age and the vasorelaxation induced by extracellular K+ was significantly higher in coronary arteries of old rats. The sensitivity (pD2) to 5-HT was significantly higher in arteries from old (6.43 ± 0.11, n = 22) than from young rats (6.16 ± 0.08, n = 29). Ketanserin induced a concentration-dependent rightward shift of the 5-HT concentration-response curve in arteries from both young and old rats. The slopes of the regression lines of the Schild plots were not significantly different from unity and the estimated pKB values for ketanserin were similar. In conclusion, ageing is associated with changes in passive mechanical characteristics as well as changes in pharmacological properties in rat coronary small arteries.
U2 - 10.1159/000341722
DO - 10.1159/000341722
M3 - Journal article
C2 - 22922218
VL - 90
SP - 160
EP - 168
JO - Pharmacology
JF - Pharmacology
SN - 0031-7012
IS - 3-4
ER -
ID: 40349736