Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs

Research output: Contribution to conference › Poster › Research › peer-review

Standard

Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs. / Ahmed, L. Sabbah; Abdolalizadeh, Bahareh; Sheykhzade, Majid; Hansen, Paul Robert.

2018. Poster session presented at Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018..

Research output: Contribution to conference › Poster › Research › peer-review

Harvard

Ahmed, LS, Abdolalizadeh, B, Sheykhzade, M & Hansen, PR 2018, 'Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs', Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018., 11/02/2018 - 16/02/2018.

APA

Ahmed, L. S., Abdolalizadeh, B., Sheykhzade, M., & Hansen, P. R. (2018). Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs. Poster session presented at Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018..

Vancouver

Ahmed LS, Abdolalizadeh B, Sheykhzade M, Hansen PR. Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs. 2018. Poster session presented at Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018..

Author

Ahmed, L. Sabbah ; Abdolalizadeh, Bahareh ; Sheykhzade, Majid ; Hansen, Paul Robert. / Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs. Poster session presented at Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018..

Bibtex

@conference{76ca06b8168a4f43861f4d74e8877e13,
title = "Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs",
abstract = "Human-alpha-Calcitonin Gene-Related Peptide (h-alpha-CGRP) is a naturally occurring 37 amino acid vasodilatory neuropeptide amide, ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAF, with a disulfide bond between residues 2 and 7. The peptide is found in primary afferent sensory nerves and is widely distributed throughout the central and peripheral nervous systems in the body.1 Structure activity studies of h-alpha-CGRP have shown that the middle and C-terminal part of the peptide allow the formation of the appropriate conformation required for the interaction with the receptor, while the N-terminus is essential for biological activity and onset of signal transduction.Fluorescent h-alpha-CGRP analogs are useful for investigating the mechanism of action behind (re)uptake of h-alpha-CGRP into the sensory nerve terminals and monitoring trafficking of CGRP receptors. As part of an ongoing study on the mechanism of action behind h-alpha-CGRP-induced vasodilation, we here present an Fmoc strategy for the synthesis of h-alpha-CGRP, and two fluorescent h-alpha-CGRP analogs labelled with 5(6)-carboxyfluorescein (Fluo) at the side-chain of K24 and K35. Pseudoprolins dipeptides were used in position 5–6, 8–9 and 16–17. Following purification by preparative HPLC, concentration-response curves were made with fluorescent CGRP analogues on isolated human subcutaneous arteries. CGRP (fluolysine35) and CGRP (wildtype) showed similar potency (pIC50 = 9.40) while CGRP (fluolysine24) showed approximately 5-fold less potency (pIC50 = 8.72) compared to the potency of CGRP (wildtype). In addition, the untagged analogue CGRP (wildtype) and commercially obtained CGRP (BACHEM) showed almost overlapping concentration-response curves.1. Sheykhzade M. et al., European Journal of Pharmacology 1998, 351(1): 53-59.2.Nilsson, C. et al. European Journal of Pharmacology, 2016, 773: 24-31.",
author = "Ahmed, {L. Sabbah} and Bahareh Abdolalizadeh and Majid Sheykhzade and Hansen, {Paul Robert}",
year = "2018",
month = feb,
day = "11",
language = "English",
note = "Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018. ; Conference date: 11-02-2018 Through 16-02-2018",

}

RIS

TY - CONF

T1 - Fmoc Solid-Phase Synthesis of Human a-Calcitonin Gene- Related Peptide and Two Carboxyfluorescein-labeled Analogs

AU - Ahmed, L. Sabbah

AU - Abdolalizadeh, Bahareh

AU - Sheykhzade, Majid

AU - Hansen, Paul Robert

PY - 2018/2/11

Y1 - 2018/2/11

N2 - Human-alpha-Calcitonin Gene-Related Peptide (h-alpha-CGRP) is a naturally occurring 37 amino acid vasodilatory neuropeptide amide, ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAF, with a disulfide bond between residues 2 and 7. The peptide is found in primary afferent sensory nerves and is widely distributed throughout the central and peripheral nervous systems in the body.1 Structure activity studies of h-alpha-CGRP have shown that the middle and C-terminal part of the peptide allow the formation of the appropriate conformation required for the interaction with the receptor, while the N-terminus is essential for biological activity and onset of signal transduction.Fluorescent h-alpha-CGRP analogs are useful for investigating the mechanism of action behind (re)uptake of h-alpha-CGRP into the sensory nerve terminals and monitoring trafficking of CGRP receptors. As part of an ongoing study on the mechanism of action behind h-alpha-CGRP-induced vasodilation, we here present an Fmoc strategy for the synthesis of h-alpha-CGRP, and two fluorescent h-alpha-CGRP analogs labelled with 5(6)-carboxyfluorescein (Fluo) at the side-chain of K24 and K35. Pseudoprolins dipeptides were used in position 5–6, 8–9 and 16–17. Following purification by preparative HPLC, concentration-response curves were made with fluorescent CGRP analogues on isolated human subcutaneous arteries. CGRP (fluolysine35) and CGRP (wildtype) showed similar potency (pIC50 = 9.40) while CGRP (fluolysine24) showed approximately 5-fold less potency (pIC50 = 8.72) compared to the potency of CGRP (wildtype). In addition, the untagged analogue CGRP (wildtype) and commercially obtained CGRP (BACHEM) showed almost overlapping concentration-response curves.1. Sheykhzade M. et al., European Journal of Pharmacology 1998, 351(1): 53-59.2.Nilsson, C. et al. European Journal of Pharmacology, 2016, 773: 24-31.

AB - Human-alpha-Calcitonin Gene-Related Peptide (h-alpha-CGRP) is a naturally occurring 37 amino acid vasodilatory neuropeptide amide, ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAF, with a disulfide bond between residues 2 and 7. The peptide is found in primary afferent sensory nerves and is widely distributed throughout the central and peripheral nervous systems in the body.1 Structure activity studies of h-alpha-CGRP have shown that the middle and C-terminal part of the peptide allow the formation of the appropriate conformation required for the interaction with the receptor, while the N-terminus is essential for biological activity and onset of signal transduction.Fluorescent h-alpha-CGRP analogs are useful for investigating the mechanism of action behind (re)uptake of h-alpha-CGRP into the sensory nerve terminals and monitoring trafficking of CGRP receptors. As part of an ongoing study on the mechanism of action behind h-alpha-CGRP-induced vasodilation, we here present an Fmoc strategy for the synthesis of h-alpha-CGRP, and two fluorescent h-alpha-CGRP analogs labelled with 5(6)-carboxyfluorescein (Fluo) at the side-chain of K24 and K35. Pseudoprolins dipeptides were used in position 5–6, 8–9 and 16–17. Following purification by preparative HPLC, concentration-response curves were made with fluorescent CGRP analogues on isolated human subcutaneous arteries. CGRP (fluolysine35) and CGRP (wildtype) showed similar potency (pIC50 = 9.40) while CGRP (fluolysine24) showed approximately 5-fold less potency (pIC50 = 8.72) compared to the potency of CGRP (wildtype). In addition, the untagged analogue CGRP (wildtype) and commercially obtained CGRP (BACHEM) showed almost overlapping concentration-response curves.1. Sheykhzade M. et al., European Journal of Pharmacology 1998, 351(1): 53-59.2.Nilsson, C. et al. European Journal of Pharmacology, 2016, 773: 24-31.

UR - https://www.grc.org/chemistry-and-biology-of-peptides-conference/2018/

M3 - Poster

T2 - Gordon Conference on Peptides, Ventura, CA, USA February 11-16, 2018.

Y2 - 11 February 2018 through 16 February 2018

ER -

ID: 188120621