A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum.

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Standard

A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum. / Nissen, Mogens Holst; Johansen, B; Bjerrum, Ole Jannik.

In: Journal of Immunological Methods, Vol. 205, No. 1, 1997, p. 29-33.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nissen, MH, Johansen, B & Bjerrum, OJ 1997, 'A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum.', Journal of Immunological Methods, vol. 205, no. 1, pp. 29-33.

APA

Nissen, M. H., Johansen, B., & Bjerrum, O. J. (1997). A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum. Journal of Immunological Methods, 205(1), 29-33.

Vancouver

Nissen MH, Johansen B, Bjerrum OJ. A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum. Journal of Immunological Methods. 1997;205(1):29-33.

Author

Nissen, Mogens Holst ; Johansen, B ; Bjerrum, Ole Jannik. / A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum. In: Journal of Immunological Methods. 1997 ; Vol. 205, No. 1. pp. 29-33.

Bibtex

@article{a7f5ae10ba3211ddae57000ea68e967b,
title = "A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum.",
abstract = "A simple method is described for the preparation of proteolytically processed forms of beta 2-microglobulin suitable for structural and biological studies. PEG 6000 was added to the serum of healthy individuals to precipitate the C1 complement complex from C1 esterase inhibitor (C1-inh). After dissolving the precipitate containing the C1 complement in Tris-HCl buffer, pH 7.6, efficient conversion of added beta 2-microglobulin to desLys58 beta 2-microglobulin was observed. Addition of a specific carboxypeptidase B inhibitor (Plummers inhibitor) could partly prevent the deletion of Lys-58 from cleaved beta 2-microglobulin, whereby Lys58-cleaved beta 2-microglobulin was obtained. The proteolytically processed forms were subsequently purified by G-75 Sephadex gel filtration followed by chromatofocusing. A yield of 10-40% of proteolytically processed beta 2-microglobulin was obtained. Only one component was seen by SDS-PAGE stained with Coomassie Brilliant Blue.",
author = "Nissen, {Mogens Holst} and B Johansen and Bjerrum, {Ole Jannik}",
note = "Keywords: Chromatography, Gel; Complement C1; Enzyme-Linked Immunosorbent Assay; Humans; beta 2-Microglobulin",
year = "1997",
language = "English",
volume = "205",
pages = "29--33",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - A simple method for the preparation and purification of C1 complement cleaved beta 2-microglobulin from human serum.

AU - Nissen, Mogens Holst

AU - Johansen, B

AU - Bjerrum, Ole Jannik

N1 - Keywords: Chromatography, Gel; Complement C1; Enzyme-Linked Immunosorbent Assay; Humans; beta 2-Microglobulin

PY - 1997

Y1 - 1997

N2 - A simple method is described for the preparation of proteolytically processed forms of beta 2-microglobulin suitable for structural and biological studies. PEG 6000 was added to the serum of healthy individuals to precipitate the C1 complement complex from C1 esterase inhibitor (C1-inh). After dissolving the precipitate containing the C1 complement in Tris-HCl buffer, pH 7.6, efficient conversion of added beta 2-microglobulin to desLys58 beta 2-microglobulin was observed. Addition of a specific carboxypeptidase B inhibitor (Plummers inhibitor) could partly prevent the deletion of Lys-58 from cleaved beta 2-microglobulin, whereby Lys58-cleaved beta 2-microglobulin was obtained. The proteolytically processed forms were subsequently purified by G-75 Sephadex gel filtration followed by chromatofocusing. A yield of 10-40% of proteolytically processed beta 2-microglobulin was obtained. Only one component was seen by SDS-PAGE stained with Coomassie Brilliant Blue.

AB - A simple method is described for the preparation of proteolytically processed forms of beta 2-microglobulin suitable for structural and biological studies. PEG 6000 was added to the serum of healthy individuals to precipitate the C1 complement complex from C1 esterase inhibitor (C1-inh). After dissolving the precipitate containing the C1 complement in Tris-HCl buffer, pH 7.6, efficient conversion of added beta 2-microglobulin to desLys58 beta 2-microglobulin was observed. Addition of a specific carboxypeptidase B inhibitor (Plummers inhibitor) could partly prevent the deletion of Lys-58 from cleaved beta 2-microglobulin, whereby Lys58-cleaved beta 2-microglobulin was obtained. The proteolytically processed forms were subsequently purified by G-75 Sephadex gel filtration followed by chromatofocusing. A yield of 10-40% of proteolytically processed beta 2-microglobulin was obtained. Only one component was seen by SDS-PAGE stained with Coomassie Brilliant Blue.

M3 - Journal article

C2 - 9236912

VL - 205

SP - 29

EP - 33

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -

ID: 8746501