Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates: Antisense Targeting of Fatty Acid Biosynthesis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates : Antisense Targeting of Fatty Acid Biosynthesis. / Hansen, Anna Mette; Bonke, Gitte; Larsen, Camilla Josephine; Yavari, Niloofar; Nielsen, Peter E.; Franzyk, Henrik.

In: Bioconjugate Chemistry, Vol. 27, No. 4, 2016, p. 863-867.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, AM, Bonke, G, Larsen, CJ, Yavari, N, Nielsen, PE & Franzyk, H 2016, 'Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates: Antisense Targeting of Fatty Acid Biosynthesis', Bioconjugate Chemistry, vol. 27, no. 4, pp. 863-867. https://doi.org/10.1021/acs.bioconjchem.6b00013

APA

Hansen, A. M., Bonke, G., Larsen, C. J., Yavari, N., Nielsen, P. E., & Franzyk, H. (2016). Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates: Antisense Targeting of Fatty Acid Biosynthesis. Bioconjugate Chemistry, 27(4), 863-867. https://doi.org/10.1021/acs.bioconjchem.6b00013

Vancouver

Hansen AM, Bonke G, Larsen CJ, Yavari N, Nielsen PE, Franzyk H. Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates: Antisense Targeting of Fatty Acid Biosynthesis. Bioconjugate Chemistry. 2016;27(4):863-867. https://doi.org/10.1021/acs.bioconjchem.6b00013

Author

Hansen, Anna Mette ; Bonke, Gitte ; Larsen, Camilla Josephine ; Yavari, Niloofar ; Nielsen, Peter E. ; Franzyk, Henrik. / Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates : Antisense Targeting of Fatty Acid Biosynthesis. In: Bioconjugate Chemistry. 2016 ; Vol. 27, No. 4. pp. 863-867.

Bibtex

@article{6b396019a1ca46adb3de7ced4d47330c,
title = "Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates: Antisense Targeting of Fatty Acid Biosynthesis",
abstract = "Antisense peptide nucleic acid (PNA) oligomers constitute a novel class of potential antibiotics that inhibit bacterial growth via specific knockdown of essential gene expression. However, discovery of efficient, nontoxic delivery vehicles for such PNA oligomers has remained a challenge. In the present study we show that antimicrobial peptides (AMPs) with an intracellular mode of action can be efficient vehicles for bacterial delivery of an antibacterial PNA targeting the essential acpP gene. The results demonstrate that buforin 2-A (BF2-A), drosocin, oncocin 10, Pep-1-K, KLW-9,13-a, (P59→W59)-Tat48-60, BF-2A-RXR, and drosocin-RXR are capable of transporting PNA effectively into E. coli (MICs of 1-4 μM). Importantly, presence of the inner-membrane peptide transporter SbmA was not required for antibacterial activity of PNA-AMP conjugates containing Pep-1-K, KLW-9,13-a, or drosocin-RXR (MICs of 2-4 μM).",
author = "Hansen, {Anna Mette} and Gitte Bonke and Larsen, {Camilla Josephine} and Niloofar Yavari and Nielsen, {Peter E.} and Henrik Franzyk",
year = "2016",
doi = "10.1021/acs.bioconjchem.6b00013",
language = "English",
volume = "27",
pages = "863--867",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "4",

}

RIS

TY - JOUR

T1 - Antibacterial Peptide Nucleic Acid-Antimicrobial Peptide (PNA-AMP) Conjugates

T2 - Antisense Targeting of Fatty Acid Biosynthesis

AU - Hansen, Anna Mette

AU - Bonke, Gitte

AU - Larsen, Camilla Josephine

AU - Yavari, Niloofar

AU - Nielsen, Peter E.

AU - Franzyk, Henrik

PY - 2016

Y1 - 2016

N2 - Antisense peptide nucleic acid (PNA) oligomers constitute a novel class of potential antibiotics that inhibit bacterial growth via specific knockdown of essential gene expression. However, discovery of efficient, nontoxic delivery vehicles for such PNA oligomers has remained a challenge. In the present study we show that antimicrobial peptides (AMPs) with an intracellular mode of action can be efficient vehicles for bacterial delivery of an antibacterial PNA targeting the essential acpP gene. The results demonstrate that buforin 2-A (BF2-A), drosocin, oncocin 10, Pep-1-K, KLW-9,13-a, (P59→W59)-Tat48-60, BF-2A-RXR, and drosocin-RXR are capable of transporting PNA effectively into E. coli (MICs of 1-4 μM). Importantly, presence of the inner-membrane peptide transporter SbmA was not required for antibacterial activity of PNA-AMP conjugates containing Pep-1-K, KLW-9,13-a, or drosocin-RXR (MICs of 2-4 μM).

AB - Antisense peptide nucleic acid (PNA) oligomers constitute a novel class of potential antibiotics that inhibit bacterial growth via specific knockdown of essential gene expression. However, discovery of efficient, nontoxic delivery vehicles for such PNA oligomers has remained a challenge. In the present study we show that antimicrobial peptides (AMPs) with an intracellular mode of action can be efficient vehicles for bacterial delivery of an antibacterial PNA targeting the essential acpP gene. The results demonstrate that buforin 2-A (BF2-A), drosocin, oncocin 10, Pep-1-K, KLW-9,13-a, (P59→W59)-Tat48-60, BF-2A-RXR, and drosocin-RXR are capable of transporting PNA effectively into E. coli (MICs of 1-4 μM). Importantly, presence of the inner-membrane peptide transporter SbmA was not required for antibacterial activity of PNA-AMP conjugates containing Pep-1-K, KLW-9,13-a, or drosocin-RXR (MICs of 2-4 μM).

U2 - 10.1021/acs.bioconjchem.6b00013

DO - 10.1021/acs.bioconjchem.6b00013

M3 - Journal article

C2 - 26938833

VL - 27

SP - 863

EP - 867

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 4

ER -

ID: 160107762