Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics : LPS and LTA Neutralization. / Skovbakke, Sarah Line; Franzyk, Henrik.

Methods Molecular Biology: Antimicrobial Peptides. ed. / Paul R. Hansen. Vol. 1548 Springer, 2017. p. 369-386 (Methods in Molecular Biology, Vol. 1548).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Skovbakke, SL & Franzyk, H 2017, Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization. in PR Hansen (ed.), Methods Molecular Biology: Antimicrobial Peptides. vol. 1548, Springer, Methods in Molecular Biology, vol. 1548, pp. 369-386.

APA

Skovbakke, S. L., & Franzyk, H. (2017). Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization. In P. R. Hansen (Ed.), Methods Molecular Biology: Antimicrobial Peptides (Vol. 1548, pp. 369-386). Springer. Methods in Molecular Biology Vol. 1548

Vancouver

Skovbakke SL, Franzyk H. Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization. In Hansen PR, editor, Methods Molecular Biology: Antimicrobial Peptides. Vol. 1548. Springer. 2017. p. 369-386. (Methods in Molecular Biology, Vol. 1548).

Author

Skovbakke, Sarah Line ; Franzyk, Henrik. / Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics : LPS and LTA Neutralization. Methods Molecular Biology: Antimicrobial Peptides. editor / Paul R. Hansen. Vol. 1548 Springer, 2017. pp. 369-386 (Methods in Molecular Biology, Vol. 1548).

Bibtex

@inbook{d764216c19874a8ba838eadd1a1d6ef1,
title = "Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization",
abstract = "Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) neutralization constitute potential non-antibiotic treatment strategies for sepsis - a systemic infection-induced inflammatory response. Studies on LPS- and LTA-neutralizing compounds are abundant in literature, and a number of peptides and peptidomimetics appear to display promising activity. However, in this ongoing search for potential antisepsis drug leads, it will be preferable that the assays used by different research groups lead to readily comparable data for the most efficient compounds. Here, we propose and describe standardized methods to be used for testing of novel compounds for their LPS- and LTA-neutralizing capacity with a focus on functional suppression of pro-inflammatory responses in cell-based systems. To best mimic the human in vivo conditions, we suggest the use of freshly isolated human leukocytes combined with an appropriate method for the chosen cytokine (e.g., IL-6 or TNF-α). The described protocols comprise isolation, stimulation, and viability test of the human leukocytes.",
author = "Skovbakke, {Sarah Line} and Henrik Franzyk",
year = "2017",
language = "English",
volume = "1548",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "369--386",
editor = "Hansen, {Paul R.}",
booktitle = "Methods Molecular Biology",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics

T2 - LPS and LTA Neutralization

AU - Skovbakke, Sarah Line

AU - Franzyk, Henrik

PY - 2017

Y1 - 2017

N2 - Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) neutralization constitute potential non-antibiotic treatment strategies for sepsis - a systemic infection-induced inflammatory response. Studies on LPS- and LTA-neutralizing compounds are abundant in literature, and a number of peptides and peptidomimetics appear to display promising activity. However, in this ongoing search for potential antisepsis drug leads, it will be preferable that the assays used by different research groups lead to readily comparable data for the most efficient compounds. Here, we propose and describe standardized methods to be used for testing of novel compounds for their LPS- and LTA-neutralizing capacity with a focus on functional suppression of pro-inflammatory responses in cell-based systems. To best mimic the human in vivo conditions, we suggest the use of freshly isolated human leukocytes combined with an appropriate method for the chosen cytokine (e.g., IL-6 or TNF-α). The described protocols comprise isolation, stimulation, and viability test of the human leukocytes.

AB - Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) neutralization constitute potential non-antibiotic treatment strategies for sepsis - a systemic infection-induced inflammatory response. Studies on LPS- and LTA-neutralizing compounds are abundant in literature, and a number of peptides and peptidomimetics appear to display promising activity. However, in this ongoing search for potential antisepsis drug leads, it will be preferable that the assays used by different research groups lead to readily comparable data for the most efficient compounds. Here, we propose and describe standardized methods to be used for testing of novel compounds for their LPS- and LTA-neutralizing capacity with a focus on functional suppression of pro-inflammatory responses in cell-based systems. To best mimic the human in vivo conditions, we suggest the use of freshly isolated human leukocytes combined with an appropriate method for the chosen cytokine (e.g., IL-6 or TNF-α). The described protocols comprise isolation, stimulation, and viability test of the human leukocytes.

UR - https://link.springer.com/protocol/10.1007/978-1-4939-6737-7_27

M3 - Book chapter

C2 - 28013519

VL - 1548

T3 - Methods in Molecular Biology

SP - 369

EP - 386

BT - Methods Molecular Biology

A2 - Hansen, Paul R.

PB - Springer

ER -

ID: 170682095