Astrocyte glycogen metabolism is required for neural activity during aglycemia or intense stimulation in mouse white matter
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Astrocyte glycogen metabolism is required for neural activity during aglycemia or intense stimulation in mouse white matter. / Brown, Angus M; Sickmann, Helle M; Fosgerau, Keld; Lund, Trine Meldgaard; Schousboe, Arne; Waagepetersen, Helle S; Ransom, Bruce R.
In: Journal of Neuroscience Research, Vol. 79, No. 1-2, 2005, p. 74-80.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Astrocyte glycogen metabolism is required for neural activity during aglycemia or intense stimulation in mouse white matter
AU - Brown, Angus M
AU - Sickmann, Helle M
AU - Fosgerau, Keld
AU - Lund, Trine Meldgaard
AU - Schousboe, Arne
AU - Waagepetersen, Helle S
AU - Ransom, Bruce R
N1 - (c) 2004 Wiley-Liss, Inc.
PY - 2005
Y1 - 2005
N2 - We tested the hypothesis that inhibiting glycogen degradation accelerates compound action potential (CAP) failure in mouse optic nerve (MON) during aglycemia or high-intensity stimulation. Axon function was assessed as the evoked CAP, and glycogen content was measured biochemically. Isofagomine, a novel inhibitor of central nervous system (CNS) glycogen phosphorylase, significantly increased glycogen content under normoglycemic conditions. When MONs were bathed in artificial cerebrospinal fluid (aCSF) containing 10 mM glucose, the CAP failed 16 min after exposure to glucose-free aCSF. MONs bathed in aCSF plus isofagomine displayed accelerated CAP failure on glucose removal. Similar results were obtained in MONs bathed in 30 mM glucose, which increased baseline glycogen concentration. The ability of isofagomine to increase glycogen content thus was not translated into delayed CAP failure. This is likely due to the inability of the tissue to metabolize glycogen in the presence of isofagomine, highlighting the importance of glycogen in sustaining neural function during aglycemia. The hypothesis that glycogen breakdown supports intense neural activity was tested by blocking glycogen breakdown during periods of high-frequency stimulation. The CAP area declined more rapidly when glycogen metabolism was inhibited by isofagomine, explicitly showing an important physiological role for glycogen metabolism during neural activity.
AB - We tested the hypothesis that inhibiting glycogen degradation accelerates compound action potential (CAP) failure in mouse optic nerve (MON) during aglycemia or high-intensity stimulation. Axon function was assessed as the evoked CAP, and glycogen content was measured biochemically. Isofagomine, a novel inhibitor of central nervous system (CNS) glycogen phosphorylase, significantly increased glycogen content under normoglycemic conditions. When MONs were bathed in artificial cerebrospinal fluid (aCSF) containing 10 mM glucose, the CAP failed 16 min after exposure to glucose-free aCSF. MONs bathed in aCSF plus isofagomine displayed accelerated CAP failure on glucose removal. Similar results were obtained in MONs bathed in 30 mM glucose, which increased baseline glycogen concentration. The ability of isofagomine to increase glycogen content thus was not translated into delayed CAP failure. This is likely due to the inability of the tissue to metabolize glycogen in the presence of isofagomine, highlighting the importance of glycogen in sustaining neural function during aglycemia. The hypothesis that glycogen breakdown supports intense neural activity was tested by blocking glycogen breakdown during periods of high-frequency stimulation. The CAP area declined more rapidly when glycogen metabolism was inhibited by isofagomine, explicitly showing an important physiological role for glycogen metabolism during neural activity.
KW - Action Potentials
KW - Analysis of Variance
KW - Animals
KW - Animals, Newborn
KW - Astrocytes
KW - Cells, Cultured
KW - Cerebellum
KW - Drug Interactions
KW - Electric Stimulation
KW - Glucose
KW - Glycogen
KW - Glycogen Phosphorylase
KW - Hyperglycemia
KW - Hypoglycemia
KW - Imino Pyranoses
KW - Male
KW - Mice
KW - Models, Biological
KW - Optic Nerve
KW - Oxygen
KW - Piperidines
KW - Reaction Time
U2 - 10.1002/jnr.20335
DO - 10.1002/jnr.20335
M3 - Journal article
C2 - 15578727
VL - 79
SP - 74
EP - 80
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 1-2
ER -
ID: 36057872