TY - JOUR

T1 - Changes in vasodilation following myocardial ischemia/reperfusion in rats

AU - Kristiansen, Sarah Brøgger

AU - Sheykhzade, Majid

AU - Edvinsson, Lars

AU - Haanes, Kristian Agmund

N1 - Copyright © 2017. Published by Elsevier Inc.

PY - 2017/11

Y1 - 2017/11

N2 - BACKGROUND: Blockage of a coronary artery, usually caused by arteriosclerosis, can lead to life threatening acute myocardial infarction. Opening with PCI (percutaneous coronary intervention), may be lifesaving, but reperfusion might exacerbate the cellular damage, and changes in the endothelium are believed to be involved in this worsened outcome.AIM: The aim of the present study was to compare endothelial dependent and independent vasodilatory effect after experimental myocardial ischemia/reperfusion (I/R).METHODS: A well-established rat model of myocardial ischemia with 24 h of reperfusion was applied, followed by a study in a wire myograph.RESULTS: Endothelial NO dependent relaxation in response to carbachol, was sensitive to arterial depolarization, and was unaffected by I/R. In contrast, Endothelial NO dependent ADPβS signalling, which was not sensitive to arterial depolarization, was significantly reduced after I/R. Following I/R, an H2O2 dependent EDH induced dilation appears in response to both of the above agonists. In addition, calcitonin gene-related peptide (CGRP) induced vasodilation was reduced.CONCLUSION: These data show that NO dependent ADPβS induced dilation is reduced after I/R. However, there is some compensation by released H2O2 causing an EDH. Combined with a loss of maximal dilation in response to CGRP, the reduced vasodilation could be an important factor in understand the exacerbated damage after I/R.

AB - BACKGROUND: Blockage of a coronary artery, usually caused by arteriosclerosis, can lead to life threatening acute myocardial infarction. Opening with PCI (percutaneous coronary intervention), may be lifesaving, but reperfusion might exacerbate the cellular damage, and changes in the endothelium are believed to be involved in this worsened outcome.AIM: The aim of the present study was to compare endothelial dependent and independent vasodilatory effect after experimental myocardial ischemia/reperfusion (I/R).METHODS: A well-established rat model of myocardial ischemia with 24 h of reperfusion was applied, followed by a study in a wire myograph.RESULTS: Endothelial NO dependent relaxation in response to carbachol, was sensitive to arterial depolarization, and was unaffected by I/R. In contrast, Endothelial NO dependent ADPβS signalling, which was not sensitive to arterial depolarization, was significantly reduced after I/R. Following I/R, an H2O2 dependent EDH induced dilation appears in response to both of the above agonists. In addition, calcitonin gene-related peptide (CGRP) induced vasodilation was reduced.CONCLUSION: These data show that NO dependent ADPβS induced dilation is reduced after I/R. However, there is some compensation by released H2O2 causing an EDH. Combined with a loss of maximal dilation in response to CGRP, the reduced vasodilation could be an important factor in understand the exacerbated damage after I/R.

KW - Journal Article

U2 - 10.1016/j.niox.2017.09.002

DO - 10.1016/j.niox.2017.09.002

M3 - Journal article

C2 - 28919322

VL - 70

SP - 68

EP - 75

JO - Nitric Oxide: Biology and Chemistry

JF - Nitric Oxide: Biology and Chemistry

SN - 1089-8603

ER -