Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B. / Moreira, José Manuel Alfonso; Holmberg, S.
In: EMBO Journal, Vol. 19, No. 24, 2000, p. 6804-6813.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B
AU - Moreira, José Manuel Alfonso
AU - Holmberg, S
N1 - Keywords: Chromatin; DNA-Binding Proteins; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Genome, Fungal; Membrane Transport Proteins; Mutation; Nuclear Proteins; Phosphate Transport Proteins; RNA Polymerase II; Repressor Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription, Genetic
PY - 2000
Y1 - 2000
N2 - The Saccharomyces cerevisiae NHP6A and NHP6B proteins are chromatin architectural factors, functionally and structurally related to the mammalian high mobility group (HMG)-1 and -2 proteins, a family of non-sequence-specific DNA binding proteins. nhp6a nhp6b mutants have various morphological defects and are defective in the induced expression of several RNA polymerase II-transcribed genes. We found that NHP6A/B proteins are also required for full induction of the yeast CHA1 gene. Importantly, CHA1 basal level expression is increased 10-fold in an nhp6a nhp6b double deletion mutant. Micrococcal nuclease and DNase I analysis of the CHA1 gene in this strain showed an open promoter structure, characteristic of the activated state of this promoter, even under non-inducing conditions. To address the possible function of the NHP6A/B proteins in chromatin-mediated gene regulation, we performed whole-genome transcriptional profiling of a Deltanhp6a Deltanhp6b yeast strain. Our results suggest that NHP6A/B proteins play an important regulatory role, repressing as well as potentiating expression of genes involved in several cellular processes, and that NHP6A/B control is exerted at the level of the individual gene.
AB - The Saccharomyces cerevisiae NHP6A and NHP6B proteins are chromatin architectural factors, functionally and structurally related to the mammalian high mobility group (HMG)-1 and -2 proteins, a family of non-sequence-specific DNA binding proteins. nhp6a nhp6b mutants have various morphological defects and are defective in the induced expression of several RNA polymerase II-transcribed genes. We found that NHP6A/B proteins are also required for full induction of the yeast CHA1 gene. Importantly, CHA1 basal level expression is increased 10-fold in an nhp6a nhp6b double deletion mutant. Micrococcal nuclease and DNase I analysis of the CHA1 gene in this strain showed an open promoter structure, characteristic of the activated state of this promoter, even under non-inducing conditions. To address the possible function of the NHP6A/B proteins in chromatin-mediated gene regulation, we performed whole-genome transcriptional profiling of a Deltanhp6a Deltanhp6b yeast strain. Our results suggest that NHP6A/B proteins play an important regulatory role, repressing as well as potentiating expression of genes involved in several cellular processes, and that NHP6A/B control is exerted at the level of the individual gene.
U2 - 10.1093/emboj/19.24.6804
DO - 10.1093/emboj/19.24.6804
M3 - Journal article
C2 - 11118215
VL - 19
SP - 6804
EP - 6813
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 24
ER -
ID: 180313