Cultured adult porcine astrocytes and microglia express functional interferon-γ receptors and exhibit toxicity towards SH-SY5Y cells
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Cultured adult porcine astrocytes and microglia express functional interferon-γ receptors and exhibit toxicity towards SH-SY5Y cells. / Ionescu, Vlad A; Villanueva, Erika B; Hashioka, Sadayuki; Bahniwal, Manpreet; Klegeris, Andis.
In: Brain Research Bulletin, Vol. 84, No. 3, 28.02.2011, p. 244-51.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Cultured adult porcine astrocytes and microglia express functional interferon-γ receptors and exhibit toxicity towards SH-SY5Y cells
AU - Ionescu, Vlad A
AU - Villanueva, Erika B
AU - Hashioka, Sadayuki
AU - Bahniwal, Manpreet
AU - Klegeris, Andis
N1 - Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.
PY - 2011/2/28
Y1 - 2011/2/28
N2 - In vitro cultures of various glial cell types are common systems used to model neuroinflammatory processes associated with age-dependent human neurodegenerative diseases. Even though most researchers choose to use neonatal rodent brain tissues as the source of glial cells, there are significant variations in glial cell functions that are species and age dependent. It has been established that human and swine immune systems have a number of similarities, which suggests that cultured porcine microglia and astrocytes may be good surrogates for human glial cell types. Here we describe a method that could be used to prepare more than 90% pure microglia and astrocyte cultures derived from adult porcine tissues. We demonstrate that both microglia and astrocytes derived from adult porcine brains express functional interferon-γ receptors (IFN-γ-R) and CD14. They become toxic towards SH-SY5Y neuroblastoma cells when exposed to proinflammatory mediators. Upon such stimulation with lipopolysaccharide (LPS) and interferon-γ (IFN-γ), adult porcine microglia, but not astrocytes, secrete tumor necrosis factor-α (TNF-α) while both cell types do not secrete detectable levels of nitric oxide (NO). Comparison of our experimental data with previously published studies indicates that adult porcine glial cultures have certain functional characteristics that make them similar to human glial cells. Therefore adult porcine glial cells may be a useful model system for studies of human diseases associated with adulthood and advanced age. Adult porcine tissues are relatively easy to obtain in most countries and could be used as a reliable and inexpensive source of cultured cells.
AB - In vitro cultures of various glial cell types are common systems used to model neuroinflammatory processes associated with age-dependent human neurodegenerative diseases. Even though most researchers choose to use neonatal rodent brain tissues as the source of glial cells, there are significant variations in glial cell functions that are species and age dependent. It has been established that human and swine immune systems have a number of similarities, which suggests that cultured porcine microglia and astrocytes may be good surrogates for human glial cell types. Here we describe a method that could be used to prepare more than 90% pure microglia and astrocyte cultures derived from adult porcine tissues. We demonstrate that both microglia and astrocytes derived from adult porcine brains express functional interferon-γ receptors (IFN-γ-R) and CD14. They become toxic towards SH-SY5Y neuroblastoma cells when exposed to proinflammatory mediators. Upon such stimulation with lipopolysaccharide (LPS) and interferon-γ (IFN-γ), adult porcine microglia, but not astrocytes, secrete tumor necrosis factor-α (TNF-α) while both cell types do not secrete detectable levels of nitric oxide (NO). Comparison of our experimental data with previously published studies indicates that adult porcine glial cultures have certain functional characteristics that make them similar to human glial cells. Therefore adult porcine glial cells may be a useful model system for studies of human diseases associated with adulthood and advanced age. Adult porcine tissues are relatively easy to obtain in most countries and could be used as a reliable and inexpensive source of cultured cells.
KW - Animals
KW - Astrocytes/immunology
KW - Cell Culture Techniques
KW - Cell Line, Tumor
KW - Cell Lineage/immunology
KW - Cells, Cultured
KW - Humans
KW - Microglia/immunology
KW - Receptors, Interferon/biosynthesis
KW - Sus scrofa
U2 - 10.1016/j.brainresbull.2010.12.011
DO - 10.1016/j.brainresbull.2010.12.011
M3 - Journal article
C2 - 21185917
VL - 84
SP - 244
EP - 251
JO - Brain Research Bulletin
JF - Brain Research Bulletin
SN - 0361-9230
IS - 3
ER -
ID: 236604320