Endocannabinoid metabolism in human glioblastomas and meningiomas compared to human non-tumour brain tissue
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The endogenous levels of the two cannabinoid receptor ligands 2-arachidonoyl glycerol and anandamide, and their respective congeners, monoacyl glycerols and N-acylethanolamines, as well as the phospholipid precursors of N-acylethanolamines, were measured by gas chromatography-mass spectrometry in glioblastoma (WHO grade IV) tissue and meningioma (WHO grade I) tissue and compared with human non-tumour brain tissue. Furthermore, the metabolic turnover of N-acylethanolamines was compared by measurements of the enzymatic activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolysing phospholipase D and fatty acid amide hydrolase in the same three types of tissue. Glioblastomas were characterized by enhanced levels of N-acylethanolamines (eightfold, 128 ± 59 pmol/µmol lipid phosphorus) including anandamide (17-fold, 4.6 ± 3.1 pmol/µmol lipid phosphorus) and several species of N-acylphosphatidylethanolamines (three to eightfold). This was accompanied by a more than 60% reduction in the enzyme activities of N- acylphosphatidylethanolamine-hydrolysing phospholipase D and fatty acid amide hydrolase. By contrast, meningiomas were characterized by a massively enhanced level of 2-monoacyl glycerols (20-fold, 2293 ± 361 pmol/µmol lipid phosphorus) including 2-arachidonoyl glycerol (20-fold, 1524 ± 361 pmol/µmol lipid phosphorus). This was accompanied by an enhanced in vitro conversion of phosphatidylcholine to monoacyl glycerol (fivefold). The enhanced level of the 2-arachidonoyl glycerol, anandamide and other N-acylethanolamines detected in the two types of tumour tissue may possibly act as endogenous anti-tumour mediators by stimulation of both cannabinoid and non-cannabinoid receptor-mediated mechanisms.
Original language | English |
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Journal | Journal of Neurochemistry |
Volume | 93 |
Issue number | 2 |
Pages (from-to) | 299-309 |
Number of pages | 11 |
ISSN | 0022-3042 |
DOIs | |
Publication status | Published - 1 Apr 2005 |
ID: 45561799