Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

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Standard

Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1. / Kulahin, Nikolaj; Kasper, Christina; Gajhede, Michael; Berezin, Vladimir; Bock, Elisabeth; Kastrup, Jette Sandholm Jensen.

In: Acta Crystallographica. Section D: Biological Crystallography, Vol. 60, No. Pt 3, 03.2004, p. 591-3.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kulahin, N, Kasper, C, Gajhede, M, Berezin, V, Bock, E & Kastrup, JSJ 2004, 'Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1', Acta Crystallographica. Section D: Biological Crystallography, vol. 60, no. Pt 3, pp. 591-3. https://doi.org/10.1107/S0907444904001167

APA

Kulahin, N., Kasper, C., Gajhede, M., Berezin, V., Bock, E., & Kastrup, J. S. J. (2004). Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1. Acta Crystallographica. Section D: Biological Crystallography, 60(Pt 3), 591-3. https://doi.org/10.1107/S0907444904001167

Vancouver

Kulahin N, Kasper C, Gajhede M, Berezin V, Bock E, Kastrup JSJ. Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1. Acta Crystallographica. Section D: Biological Crystallography. 2004 Mar;60(Pt 3):591-3. https://doi.org/10.1107/S0907444904001167

Author

Kulahin, Nikolaj ; Kasper, Christina ; Gajhede, Michael ; Berezin, Vladimir ; Bock, Elisabeth ; Kastrup, Jette Sandholm Jensen. / Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1. In: Acta Crystallographica. Section D: Biological Crystallography. 2004 ; Vol. 60, No. Pt 3. pp. 591-3.

Bibtex

@article{95a07ca63a754dab89b2ba46a0f36e5d,
title = "Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1",
abstract = "Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.",
keywords = "Animals, Cell Line, Crystallization, Crystallography, X-Ray, Drosophila, Gene Expression, Mice, Neural Cell Adhesion Molecule L1, Protein Structure, Tertiary, Rats, Recombinant Proteins",
author = "Nikolaj Kulahin and Christina Kasper and Michael Gajhede and Vladimir Berezin and Elisabeth Bock and Kastrup, {Jette Sandholm Jensen}",
year = "2004",
month = mar,
doi = "10.1107/S0907444904001167",
language = "English",
volume = "60",
pages = "591--3",
journal = "Acta Crystallographica Section D: Structural Biology",
issn = "2059-7983",
publisher = "International Union of Crystallography",
number = "Pt 3",

}

RIS

TY - JOUR

T1 - Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

AU - Kulahin, Nikolaj

AU - Kasper, Christina

AU - Gajhede, Michael

AU - Berezin, Vladimir

AU - Bock, Elisabeth

AU - Kastrup, Jette Sandholm Jensen

PY - 2004/3

Y1 - 2004/3

N2 - Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.

AB - Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.

KW - Animals

KW - Cell Line

KW - Crystallization

KW - Crystallography, X-Ray

KW - Drosophila

KW - Gene Expression

KW - Mice

KW - Neural Cell Adhesion Molecule L1

KW - Protein Structure, Tertiary

KW - Rats

KW - Recombinant Proteins

U2 - 10.1107/S0907444904001167

DO - 10.1107/S0907444904001167

M3 - Journal article

C2 - 14993704

VL - 60

SP - 591

EP - 593

JO - Acta Crystallographica Section D: Structural Biology

JF - Acta Crystallographica Section D: Structural Biology

SN - 2059-7983

IS - Pt 3

ER -

ID: 44729822