Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line

Research output: Contribution to journal › Editorial › Research › peer-review

Standard

Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. / Schmid, Benjamin; Prehn, Kennie R.; Nimsanor, Natakarn; Garcia, Blanca Irene Aldana; Poulsen, Ulla; Jorring, Ida; Rasmussen, Mikkel A.; Clausen, Christian; Mau-Holzmann, Ulrike A.; Ramakrishna, Sarayu; Muddashetty, Ravi; Steeg, Rachel; Bruce, Kevin; Mackintosh, Peter; Ebneth, Andreas; Holst, Bjorn; Cabrera-Socorro, Alfredo.

In: Stem Cell Research, Vol. 34, 101349, 2019.

Research output: Contribution to journal › Editorial › Research › peer-review

Harvard

Schmid, B, Prehn, KR, Nimsanor, N, Garcia, BIA, Poulsen, U, Jorring, I, Rasmussen, MA, Clausen, C, Mau-Holzmann, UA, Ramakrishna, S, Muddashetty, R, Steeg, R, Bruce, K, Mackintosh, P, Ebneth, A, Holst, B & Cabrera-Socorro, A 2019, 'Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line', Stem Cell Research, vol. 34, 101349. https://doi.org/10.1016/j.scr.2018.11.010

APA

Schmid, B., Prehn, K. R., Nimsanor, N., Garcia, B. I. A., Poulsen, U., Jorring, I., Rasmussen, M. A., Clausen, C., Mau-Holzmann, U. A., Ramakrishna, S., Muddashetty, R., Steeg, R., Bruce, K., Mackintosh, P., Ebneth, A., Holst, B., & Cabrera-Socorro, A. (2019). Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. Stem Cell Research, 34, [101349]. https://doi.org/10.1016/j.scr.2018.11.010

Vancouver

Schmid B, Prehn KR, Nimsanor N, Garcia BIA, Poulsen U, Jorring I et al. Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. Stem Cell Research. 2019;34. 101349. https://doi.org/10.1016/j.scr.2018.11.010

Author

Schmid, Benjamin ; Prehn, Kennie R. ; Nimsanor, Natakarn ; Garcia, Blanca Irene Aldana ; Poulsen, Ulla ; Jorring, Ida ; Rasmussen, Mikkel A. ; Clausen, Christian ; Mau-Holzmann, Ulrike A. ; Ramakrishna, Sarayu ; Muddashetty, Ravi ; Steeg, Rachel ; Bruce, Kevin ; Mackintosh, Peter ; Ebneth, Andreas ; Holst, Bjorn ; Cabrera-Socorro, Alfredo. / Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line. In: Stem Cell Research. 2019 ; Vol. 34.

Bibtex

@article{5cff06d4f4744018a050bc759bcf8d87,
title = "Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line",
abstract = "Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-epsilon 2, APOE-epsilon 3 and APOE-epsilon 4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-epsilon 3/epsilon 4 genotype to obtain isogenic APOE-epsilon 2/epsilon 2, APOE-epsilon 3/epsilon 3, APOE-epsilon 4/epsilon 4 lines as well as an APOE-knock-out line.",
author = "Benjamin Schmid and Prehn, {Kennie R.} and Natakarn Nimsanor and Garcia, {Blanca Irene Aldana} and Ulla Poulsen and Ida Jorring and Rasmussen, {Mikkel A.} and Christian Clausen and Mau-Holzmann, {Ulrike A.} and Sarayu Ramakrishna and Ravi Muddashetty and Rachel Steeg and Kevin Bruce and Peter Mackintosh and Andreas Ebneth and Bjorn Holst and Alfredo Cabrera-Socorro",
note = "Corrigendum to “Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line” [Stem Cell Res. 34/1873–5061 (2019) 101349–55] Stem Cell Research, Volume 48, October 2020, Pages 102005",
year = "2019",
doi = "10.1016/j.scr.2018.11.010",
language = "English",
volume = "34",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line

AU - Schmid, Benjamin

AU - Prehn, Kennie R.

AU - Nimsanor, Natakarn

AU - Garcia, Blanca Irene Aldana

AU - Poulsen, Ulla

AU - Jorring, Ida

AU - Rasmussen, Mikkel A.

AU - Clausen, Christian

AU - Mau-Holzmann, Ulrike A.

AU - Ramakrishna, Sarayu

AU - Muddashetty, Ravi

AU - Steeg, Rachel

AU - Bruce, Kevin

AU - Mackintosh, Peter

AU - Ebneth, Andreas

AU - Holst, Bjorn

AU - Cabrera-Socorro, Alfredo

N1 - Corrigendum to “Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line” [Stem Cell Res. 34/1873–5061 (2019) 101349–55] Stem Cell Research, Volume 48, October 2020, Pages 102005

PY - 2019

Y1 - 2019

N2 - Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-epsilon 2, APOE-epsilon 3 and APOE-epsilon 4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-epsilon 3/epsilon 4 genotype to obtain isogenic APOE-epsilon 2/epsilon 2, APOE-epsilon 3/epsilon 3, APOE-epsilon 4/epsilon 4 lines as well as an APOE-knock-out line.

AB - Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-epsilon 2, APOE-epsilon 3 and APOE-epsilon 4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-epsilon 3/epsilon 4 genotype to obtain isogenic APOE-epsilon 2/epsilon 2, APOE-epsilon 3/epsilon 3, APOE-epsilon 4/epsilon 4 lines as well as an APOE-knock-out line.

U2 - 10.1016/j.scr.2018.11.010

DO - 10.1016/j.scr.2018.11.010

M3 - Editorial

VL - 34

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 101349

ER -

ID: 254733766