Glutamate signalling and secretory phospholipase A2 modulate the release of arachidonic acid from neuronal membranes
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Glutamate signalling and secretory phospholipase A2 modulate the release of arachidonic acid from neuronal membranes. / Rodriguez De Turco, Elena B; Jackson, Fannie R; DeCoster, Mark A; Kolko, Miriam; Kolko, Miriam; Bazan, Nicolas G.
In: Journal of Neuroscience Research, Vol. 68, No. 5, 01.06.2002, p. 558-67.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Glutamate signalling and secretory phospholipase A2 modulate the release of arachidonic acid from neuronal membranes
AU - Rodriguez De Turco, Elena B
AU - Jackson, Fannie R
AU - DeCoster, Mark A
AU - Kolko, Miriam
AU - Kolko, Miriam
AU - Bazan, Nicolas G
N1 - Copyright 2002 Wiley-Liss, Inc.
PY - 2002/6/1
Y1 - 2002/6/1
N2 - The lipid mediators generated by phospholipases A(2) (PLA(2)), free arachidonic acid (AA), eicosanoids, and platelet-activating factor, modulate neuronal activity; when overproduced, some of them become potent neurotoxins. We have shown, using primary cortical neuron cultures, that glutamate and secretory PLA(2) (sPLA(2)) from bee venom (bv sPLA(2)) and Taipan snake venom (OS2) elicit synergy in inducing neuronal cell death. Low concentrations of sPLA(2) are selective ligands of cell-surface sPLA(2) receptors. We investigated which neuronal arachidonoyl phospholipids are targeted by glutamate-activated cytosolic calcium-dependent PLA(2) (cPLA(2)) and by sPLA(2). Treatment of (3)H-AA-labeled cortical neurons with mildly toxic concentrations of sPLA(2) (25 ng/ml, 1.78 nM) for 45 min resulted in a two- to threefold higher loss of (3)H-AA from phosphatidylcholine (PC) than from phosphatidylethanolamine (PE) and in minor changes in other phospholipids. A similar profile, although of greater magnitude, was observed 20 hr posttreatment. Glutamate (80 microM) induced much less mobilization of (3)H-AA than did sPLA(2) and resulted in a threefold greater degradation of (3)H-AA PE than of (3)H-AA PC by 20 hr posttreatment. Combining sPLA(2) and glutamate resulted in a greater degradation of PC and PE, and the N-methyl-D-aspartate receptor antagonist MK-801 only blocked glutamate effects. Thus, activation of the arachidonate cascade induced by glutamate and sPLA(2) under experimental conditions that lead to neuronal cell death involves the hydrolysis of different (perhaps partially overlapping) cellular phospholipid pools.
AB - The lipid mediators generated by phospholipases A(2) (PLA(2)), free arachidonic acid (AA), eicosanoids, and platelet-activating factor, modulate neuronal activity; when overproduced, some of them become potent neurotoxins. We have shown, using primary cortical neuron cultures, that glutamate and secretory PLA(2) (sPLA(2)) from bee venom (bv sPLA(2)) and Taipan snake venom (OS2) elicit synergy in inducing neuronal cell death. Low concentrations of sPLA(2) are selective ligands of cell-surface sPLA(2) receptors. We investigated which neuronal arachidonoyl phospholipids are targeted by glutamate-activated cytosolic calcium-dependent PLA(2) (cPLA(2)) and by sPLA(2). Treatment of (3)H-AA-labeled cortical neurons with mildly toxic concentrations of sPLA(2) (25 ng/ml, 1.78 nM) for 45 min resulted in a two- to threefold higher loss of (3)H-AA from phosphatidylcholine (PC) than from phosphatidylethanolamine (PE) and in minor changes in other phospholipids. A similar profile, although of greater magnitude, was observed 20 hr posttreatment. Glutamate (80 microM) induced much less mobilization of (3)H-AA than did sPLA(2) and resulted in a threefold greater degradation of (3)H-AA PE than of (3)H-AA PC by 20 hr posttreatment. Combining sPLA(2) and glutamate resulted in a greater degradation of PC and PE, and the N-methyl-D-aspartate receptor antagonist MK-801 only blocked glutamate effects. Thus, activation of the arachidonate cascade induced by glutamate and sPLA(2) under experimental conditions that lead to neuronal cell death involves the hydrolysis of different (perhaps partially overlapping) cellular phospholipid pools.
KW - Animals
KW - Arachidonic Acid
KW - Cell Membrane
KW - Cells, Cultured
KW - Cerebral Cortex
KW - Glutamic Acid
KW - Neurons
KW - Phosphatidylcholines
KW - Phosphatidylethanolamines
KW - Phospholipases A
KW - Phospholipases A2
KW - Rats
KW - Signal Transduction
KW - Tritium
U2 - 10.1002/jnr.10239
DO - 10.1002/jnr.10239
M3 - Journal article
C2 - 12111845
VL - 68
SP - 558
EP - 567
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 5
ER -
ID: 128615091