How to Choose the Right Inducible Gene Expression System for Mammalian Studies?

Research output: Contribution to journal › Review › Research › peer-review

Standard

How to Choose the Right Inducible Gene Expression System for Mammalian Studies? / Kallunki, Tuula; Barisic, Marin; Jäättelä, Marja; Liu, Bin.

In: Cells, Vol. 8, No. 8, 796, 2019.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Kallunki, T, Barisic, M, Jäättelä, M & Liu, B 2019, 'How to Choose the Right Inducible Gene Expression System for Mammalian Studies?', Cells, vol. 8, no. 8, 796. https://doi.org/10.3390/cells8080796

APA

Kallunki, T., Barisic, M., Jäättelä, M., & Liu, B. (2019). How to Choose the Right Inducible Gene Expression System for Mammalian Studies? Cells, 8(8), [796]. https://doi.org/10.3390/cells8080796

Vancouver

Kallunki T, Barisic M, Jäättelä M, Liu B. How to Choose the Right Inducible Gene Expression System for Mammalian Studies? Cells. 2019;8(8). 796. https://doi.org/10.3390/cells8080796

Author

Kallunki, Tuula ; Barisic, Marin ; Jäättelä, Marja ; Liu, Bin. / How to Choose the Right Inducible Gene Expression System for Mammalian Studies?. In: Cells. 2019 ; Vol. 8, No. 8.

Bibtex

@article{a4f55479771f44eca7c2a5460af6007a,
title = "How to Choose the Right Inducible Gene Expression System for Mammalian Studies?",
abstract = "Inducible gene expression systems are favored over stable expression systems in a wide variety of basic and applied research areas, including functional genomics, gene therapy, tissue engineering, biopharmaceutical protein production and drug discovery. This is because they are mostly reversible and thus more flexible to use. Furthermore, compared to constitutive expression, they generally exhibit a higher efficiency and have fewer side effects, such as cell death and delayed growth or development. Empowered by decades of development of inducible gene expression systems, researchers can now efficiently activate or suppress any gene, temporarily and quantitively at will, depending on experimental requirements and designs. Here, we review a number of most commonly used mammalian inducible expression systems and provide basic standards and criteria for the selection of the most suitable one.",
author = "Tuula Kallunki and Marin Barisic and Marja J{\"a}{\"a}ttel{\"a} and Bin Liu",
year = "2019",
doi = "10.3390/cells8080796",
language = "English",
volume = "8",
journal = "Cells",
issn = "2073-4409",
publisher = "MDPI AG",
number = "8",

}

RIS

TY - JOUR

T1 - How to Choose the Right Inducible Gene Expression System for Mammalian Studies?

AU - Kallunki, Tuula

AU - Barisic, Marin

AU - Jäättelä, Marja

AU - Liu, Bin

PY - 2019

Y1 - 2019

N2 - Inducible gene expression systems are favored over stable expression systems in a wide variety of basic and applied research areas, including functional genomics, gene therapy, tissue engineering, biopharmaceutical protein production and drug discovery. This is because they are mostly reversible and thus more flexible to use. Furthermore, compared to constitutive expression, they generally exhibit a higher efficiency and have fewer side effects, such as cell death and delayed growth or development. Empowered by decades of development of inducible gene expression systems, researchers can now efficiently activate or suppress any gene, temporarily and quantitively at will, depending on experimental requirements and designs. Here, we review a number of most commonly used mammalian inducible expression systems and provide basic standards and criteria for the selection of the most suitable one.

AB - Inducible gene expression systems are favored over stable expression systems in a wide variety of basic and applied research areas, including functional genomics, gene therapy, tissue engineering, biopharmaceutical protein production and drug discovery. This is because they are mostly reversible and thus more flexible to use. Furthermore, compared to constitutive expression, they generally exhibit a higher efficiency and have fewer side effects, such as cell death and delayed growth or development. Empowered by decades of development of inducible gene expression systems, researchers can now efficiently activate or suppress any gene, temporarily and quantitively at will, depending on experimental requirements and designs. Here, we review a number of most commonly used mammalian inducible expression systems and provide basic standards and criteria for the selection of the most suitable one.

U2 - 10.3390/cells8080796

DO - 10.3390/cells8080796

M3 - Review

C2 - 31366153

VL - 8

JO - Cells

JF - Cells

SN - 2073-4409

IS - 8

M1 - 796

ER -

ID: 237105902