Illuminating the structure and function of Cys-loop receptors
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Illuminating the structure and function of Cys-loop receptors. / Pless, Stephan Alexander; Lynch, Joseph W.
In: Clinical and Experimental Pharmacology & Physiology (Online), Vol. 35, No. 10, 10.2008, p. 1137-42.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Illuminating the structure and function of Cys-loop receptors
AU - Pless, Stephan Alexander
AU - Lynch, Joseph W
PY - 2008/10
Y1 - 2008/10
N2 - Cys-loop receptors are an important class of ligand-gated ion channels. They mediate fast synaptic neurotransmission, are implicated in various 'channelopathies' and are important pharmacological targets. Recent progress in X-ray crystallography and electron microscopy has provided a considerable insight into the structure of Cys-loop receptors. However, data from these experiments only provide 'snapshots' of the proteins under investigation. They cannot provide information about the various conformations the protein adopts during transition from the closed to the open and desensitized states. Voltage-clamp fluorometry helps overcome this problem by simultaneously monitoring movements at the channel gate (through changes in current) and conformational rearrangements in a domain of interest (through changes in fluorescence) in real time. Thus, the technique can provide information on both transitional and steady state conformations and serves as a real time correlate of the channel structure and its function. Voltage-clamp fluorometry experiments on Cys-loop receptors have yielded a large body of data concerning the mechanisms by which agonists, antagonists and modulators act on these receptors. They have shed new light on the conformational mobility of both the ligand-binding and the transmembrane domain of Cys-loop receptors.
AB - Cys-loop receptors are an important class of ligand-gated ion channels. They mediate fast synaptic neurotransmission, are implicated in various 'channelopathies' and are important pharmacological targets. Recent progress in X-ray crystallography and electron microscopy has provided a considerable insight into the structure of Cys-loop receptors. However, data from these experiments only provide 'snapshots' of the proteins under investigation. They cannot provide information about the various conformations the protein adopts during transition from the closed to the open and desensitized states. Voltage-clamp fluorometry helps overcome this problem by simultaneously monitoring movements at the channel gate (through changes in current) and conformational rearrangements in a domain of interest (through changes in fluorescence) in real time. Thus, the technique can provide information on both transitional and steady state conformations and serves as a real time correlate of the channel structure and its function. Voltage-clamp fluorometry experiments on Cys-loop receptors have yielded a large body of data concerning the mechanisms by which agonists, antagonists and modulators act on these receptors. They have shed new light on the conformational mobility of both the ligand-binding and the transmembrane domain of Cys-loop receptors.
KW - Animals
KW - Cysteine
KW - Fluorometry
KW - Humans
KW - Ion Channel Gating
KW - Ion Channels
KW - Membrane Proteins
KW - Patch-Clamp Techniques
U2 - 10.1111/j.1440-1681.2008.04954.x
DO - 10.1111/j.1440-1681.2008.04954.x
M3 - Journal article
C2 - 18505452
VL - 35
SP - 1137
EP - 1142
JO - Clinical and Experimental Pharmacology and Physiology
JF - Clinical and Experimental Pharmacology and Physiology
SN - 0305-1870
IS - 10
ER -
ID: 122597879