In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes. / Vermehren, C.; Clausen-Beck, B.; Frøkjær, S.; Hansen, Harald S.

In: International Journal of Pharmaceutics, Vol. 254, No. 1, 18.03.2003, p. 49-53.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vermehren, C, Clausen-Beck, B, Frøkjær, S & Hansen, HS 2003, 'In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes', International Journal of Pharmaceutics, vol. 254, no. 1, pp. 49-53. https://doi.org/10.1016/S0378-5173(02)00681-6

APA

Vermehren, C., Clausen-Beck, B., Frøkjær, S., & Hansen, H. S. (2003). In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes. International Journal of Pharmaceutics, 254(1), 49-53. https://doi.org/10.1016/S0378-5173(02)00681-6

Vancouver

Vermehren C, Clausen-Beck B, Frøkjær S, Hansen HS. In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes. International Journal of Pharmaceutics. 2003 Mar 18;254(1):49-53. https://doi.org/10.1016/S0378-5173(02)00681-6

Author

Vermehren, C. ; Clausen-Beck, B. ; Frøkjær, S. ; Hansen, Harald S. / In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes. In: International Journal of Pharmaceutics. 2003 ; Vol. 254, No. 1. pp. 49-53.

Bibtex

@article{ecfcf3e3c0c4403fa2d6e23b06b0166b,
title = "In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes",
abstract = "Incorporation of the phospholipid, N-acyl-phosphatidylethanolamine (NAPE), has shown to increase the liposomal stability towards plasma components in vitro. Besides increasing the circulation-time, NAPE has been shown to contain fusiogenic properties. Hence, fusion between NAPE-liposomes and target cells may be expected, resulting in a favorable delivery of drug to the target cell. In this study, NAPE has been tested as a potential liposomal component of phosphatidylcholine-liposomes. The liposomes were characterized by size, long-term stability and phase transition temperature (T). In vivo behavior of NAPE-liposomes was determined by the blood-circulation half-life in mice. A characterization of the liposomes revealed that high content of NAPE resulted in liposomes of increased size compared to pure phosphatidylcholine-liposomes. However, the liposomes showed only a slight increase in size during storage for 5 weeks. Determination of T for NAPE-liposomes showed increasing values of T by increasing percentage of NAPE in the liposomal bilayer, due to the higher T of NAPE compared to phosphatidylcholine. Blood-clearance studies showed an initial increase in blood-circulation of liposomes containing high amounts of NAPE. Thus, these results suggest that liposomes containing high percentage of NAPE may be a promising candidate for long-circulating liposomes, possibly in combination with other stabilizing components, e.g. cholesterol.",
author = "C. Vermehren and B. Clausen-Beck and S. Fr{\o}kj{\ae}r and Hansen, {Harald S.}",
year = "2003",
month = mar,
day = "18",
doi = "10.1016/S0378-5173(02)00681-6",
language = "English",
volume = "254",
pages = "49--53",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - In vitro and in vivo aspects of N-acyl-phosphatidylethanolamine-containing liposomes

AU - Vermehren, C.

AU - Clausen-Beck, B.

AU - Frøkjær, S.

AU - Hansen, Harald S.

PY - 2003/3/18

Y1 - 2003/3/18

N2 - Incorporation of the phospholipid, N-acyl-phosphatidylethanolamine (NAPE), has shown to increase the liposomal stability towards plasma components in vitro. Besides increasing the circulation-time, NAPE has been shown to contain fusiogenic properties. Hence, fusion between NAPE-liposomes and target cells may be expected, resulting in a favorable delivery of drug to the target cell. In this study, NAPE has been tested as a potential liposomal component of phosphatidylcholine-liposomes. The liposomes were characterized by size, long-term stability and phase transition temperature (T). In vivo behavior of NAPE-liposomes was determined by the blood-circulation half-life in mice. A characterization of the liposomes revealed that high content of NAPE resulted in liposomes of increased size compared to pure phosphatidylcholine-liposomes. However, the liposomes showed only a slight increase in size during storage for 5 weeks. Determination of T for NAPE-liposomes showed increasing values of T by increasing percentage of NAPE in the liposomal bilayer, due to the higher T of NAPE compared to phosphatidylcholine. Blood-clearance studies showed an initial increase in blood-circulation of liposomes containing high amounts of NAPE. Thus, these results suggest that liposomes containing high percentage of NAPE may be a promising candidate for long-circulating liposomes, possibly in combination with other stabilizing components, e.g. cholesterol.

AB - Incorporation of the phospholipid, N-acyl-phosphatidylethanolamine (NAPE), has shown to increase the liposomal stability towards plasma components in vitro. Besides increasing the circulation-time, NAPE has been shown to contain fusiogenic properties. Hence, fusion between NAPE-liposomes and target cells may be expected, resulting in a favorable delivery of drug to the target cell. In this study, NAPE has been tested as a potential liposomal component of phosphatidylcholine-liposomes. The liposomes were characterized by size, long-term stability and phase transition temperature (T). In vivo behavior of NAPE-liposomes was determined by the blood-circulation half-life in mice. A characterization of the liposomes revealed that high content of NAPE resulted in liposomes of increased size compared to pure phosphatidylcholine-liposomes. However, the liposomes showed only a slight increase in size during storage for 5 weeks. Determination of T for NAPE-liposomes showed increasing values of T by increasing percentage of NAPE in the liposomal bilayer, due to the higher T of NAPE compared to phosphatidylcholine. Blood-clearance studies showed an initial increase in blood-circulation of liposomes containing high amounts of NAPE. Thus, these results suggest that liposomes containing high percentage of NAPE may be a promising candidate for long-circulating liposomes, possibly in combination with other stabilizing components, e.g. cholesterol.

UR - http://www.scopus.com/inward/record.url?scp=0037453171&partnerID=8YFLogxK

U2 - 10.1016/S0378-5173(02)00681-6

DO - 10.1016/S0378-5173(02)00681-6

M3 - Journal article

AN - SCOPUS:0037453171

VL - 254

SP - 49

EP - 53

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1

ER -

ID: 45562877