Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. / Midtgaard, Søren Roi; Darwish, Tamim A; Pedersen, Martin Cramer; Huda, Pie; Larsen, Andreas Haahr; Jensen, Grethe Vestergaard; Kynde, Søren Andreas Røssell; Skar-Gislinge, Nicholas; Nielsen, Agnieszka Janina Zygadlo; Olesen, Claus; Blaise, Mickael; Dorosz, Jerzy Jòzef; Thorsen, Thor Seneca; Venskutonytė, Raminta; Krintel, Christian; Møller, Jesper V.; Frielinghaus, Henrich; Gilbert, Elliot Paul; Martel, Anne; Kastrup, Jette Sandholm Jensen; Jensen, Poul Erik; Nissen, Poul; Arleth, Lise.

In: F E B S Journal, Vol. 285, No. 2, 01.2018, p. 357-371.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Midtgaard, SR, Darwish, TA, Pedersen, MC, Huda, P, Larsen, AH, Jensen, GV, Kynde, SAR, Skar-Gislinge, N, Nielsen, AJZ, Olesen, C, Blaise, M, Dorosz, JJ, Thorsen, TS, Venskutonytė, R, Krintel, C, Møller, JV, Frielinghaus, H, Gilbert, EP, Martel, A, Kastrup, JSJ, Jensen, PE, Nissen, P & Arleth, L 2018, 'Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering', F E B S Journal, vol. 285, no. 2, pp. 357-371. https://doi.org/10.1111/febs.14345

APA

Midtgaard, S. R., Darwish, T. A., Pedersen, M. C., Huda, P., Larsen, A. H., Jensen, G. V., Kynde, S. A. R., Skar-Gislinge, N., Nielsen, A. J. Z., Olesen, C., Blaise, M., Dorosz, J. J., Thorsen, T. S., Venskutonytė, R., Krintel, C., Møller, J. V., Frielinghaus, H., Gilbert, E. P., Martel, A., ... Arleth, L. (2018). Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. F E B S Journal, 285(2), 357-371. https://doi.org/10.1111/febs.14345

Vancouver

Midtgaard SR, Darwish TA, Pedersen MC, Huda P, Larsen AH, Jensen GV et al. Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. F E B S Journal. 2018 Jan;285(2):357-371. https://doi.org/10.1111/febs.14345

Author

Midtgaard, Søren Roi ; Darwish, Tamim A ; Pedersen, Martin Cramer ; Huda, Pie ; Larsen, Andreas Haahr ; Jensen, Grethe Vestergaard ; Kynde, Søren Andreas Røssell ; Skar-Gislinge, Nicholas ; Nielsen, Agnieszka Janina Zygadlo ; Olesen, Claus ; Blaise, Mickael ; Dorosz, Jerzy Jòzef ; Thorsen, Thor Seneca ; Venskutonytė, Raminta ; Krintel, Christian ; Møller, Jesper V. ; Frielinghaus, Henrich ; Gilbert, Elliot Paul ; Martel, Anne ; Kastrup, Jette Sandholm Jensen ; Jensen, Poul Erik ; Nissen, Poul ; Arleth, Lise. / Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering. In: F E B S Journal. 2018 ; Vol. 285, No. 2. pp. 357-371.

Bibtex

@article{80a8bd4f22d9452498ca50c35822474b,
title = "Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering",
abstract = "A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.",
keywords = "Journal Article",
author = "Midtgaard, {S{\o}ren Roi} and Darwish, {Tamim A} and Pedersen, {Martin Cramer} and Pie Huda and Larsen, {Andreas Haahr} and Jensen, {Grethe Vestergaard} and Kynde, {S{\o}ren Andreas R{\o}ssell} and Nicholas Skar-Gislinge and Nielsen, {Agnieszka Janina Zygadlo} and Claus Olesen and Mickael Blaise and Dorosz, {Jerzy J{\`o}zef} and Thorsen, {Thor Seneca} and Raminta Venskutonytė and Christian Krintel and M{\o}ller, {Jesper V.} and Henrich Frielinghaus and Gilbert, {Elliot Paul} and Anne Martel and Kastrup, {Jette Sandholm Jensen} and Jensen, {Poul Erik} and Poul Nissen and Lise Arleth",
note = "This article is protected by copyright. All rights reserved.",
year = "2018",
month = jan,
doi = "10.1111/febs.14345",
language = "English",
volume = "285",
pages = "357--371",
journal = "F E B S Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

AU - Midtgaard, Søren Roi

AU - Darwish, Tamim A

AU - Pedersen, Martin Cramer

AU - Huda, Pie

AU - Larsen, Andreas Haahr

AU - Jensen, Grethe Vestergaard

AU - Kynde, Søren Andreas Røssell

AU - Skar-Gislinge, Nicholas

AU - Nielsen, Agnieszka Janina Zygadlo

AU - Olesen, Claus

AU - Blaise, Mickael

AU - Dorosz, Jerzy Jòzef

AU - Thorsen, Thor Seneca

AU - Venskutonytė, Raminta

AU - Krintel, Christian

AU - Møller, Jesper V.

AU - Frielinghaus, Henrich

AU - Gilbert, Elliot Paul

AU - Martel, Anne

AU - Kastrup, Jette Sandholm Jensen

AU - Jensen, Poul Erik

AU - Nissen, Poul

AU - Arleth, Lise

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/1

Y1 - 2018/1

N2 - A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.

AB - A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.

KW - Journal Article

U2 - 10.1111/febs.14345

DO - 10.1111/febs.14345

M3 - Journal article

C2 - 29178440

VL - 285

SP - 357

EP - 371

JO - F E B S Journal

JF - F E B S Journal

SN - 1742-464X

IS - 2

ER -

ID: 186869807