From a single leaf of a herbarium sheet of Myxopyrum smilacifolium was isolated the iridoid glucosides myxopyroside (1) and its 6-O-acetyl-7-O-(E/Z)-p-methoxycinnamoyl esters (2/3) of dimethyl forsythide. The presence of these compounds indicates a close relationship with the genus Nyctanthes, from which similar compounds have earlier been reported. This finding corroborates relationships recently found within the family Oleaceae by modern gene sequencing techniques.