Membrane transport of anandamide through resealed human red blood cell membranes
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Membrane transport of anandamide through resealed human red blood cell membranes. / Bojesen, I.N.; Hansen, Harald S.
In: Journal of Lipid Research, Vol. 46, No. 8, 01.08.2005, p. 1652-1659.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Membrane transport of anandamide through resealed human red blood cell membranes
AU - Bojesen, I.N.
AU - Hansen, Harald S.
PY - 2005/8/1
Y1 - 2005/8/1
N2 - The use of resealed red blood cell membranes (ghosts) allows the study of the transport of a compound in a nonmetabolizing system with a biological membrane. Transmembrane movements of anandamide (N-arachidonoylethanolamine, arachidonoylethanolamide) have been studied by exchange efflux experiments at 0°C and pH 7.3 with albumin-free and albumin-filled human red blood cell ghosts. The efflux kinetics is biexponential and is analyzed in terms of compartment models. The distribution of anandamide on the membrane inner to outer leaflet pools is determined to be 0.275 ± 0.023, and the rate constant of unidirectional flux from inside to outside is 0.361 ± 0.023 s. The rate constant of unidirectional flux from the membrane to BSA in the medium ([BSA]) increases with the square root of [BSA] in accordance with the theory of an unstirred layer around ghosts. Anandamide passed through the red blood cell membrane very rapidly, within seconds. At a molar ratio of anandamide to BSA of
AB - The use of resealed red blood cell membranes (ghosts) allows the study of the transport of a compound in a nonmetabolizing system with a biological membrane. Transmembrane movements of anandamide (N-arachidonoylethanolamine, arachidonoylethanolamide) have been studied by exchange efflux experiments at 0°C and pH 7.3 with albumin-free and albumin-filled human red blood cell ghosts. The efflux kinetics is biexponential and is analyzed in terms of compartment models. The distribution of anandamide on the membrane inner to outer leaflet pools is determined to be 0.275 ± 0.023, and the rate constant of unidirectional flux from inside to outside is 0.361 ± 0.023 s. The rate constant of unidirectional flux from the membrane to BSA in the medium ([BSA]) increases with the square root of [BSA] in accordance with the theory of an unstirred layer around ghosts. Anandamide passed through the red blood cell membrane very rapidly, within seconds. At a molar ratio of anandamide to BSA of
UR - http://www.scopus.com/inward/record.url?scp=23244446141&partnerID=8YFLogxK
U2 - 10.1194/jlr.M400498-JLR200
DO - 10.1194/jlr.M400498-JLR200
M3 - Journal article
AN - SCOPUS:23244446141
VL - 46
SP - 1652
EP - 1659
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 8
ER -
ID: 45563139