Physiological response in E. coli to YdgR overexpression depends on whether the protein has an intact function
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Physiological response in E. coli to YdgR overexpression depends on whether the protein has an intact function. / Sajid, Salvia; Salas, Lilia Hernandez; Rafiq, Maria; Lund, Torben; Jørgensen, Mikkel Girke; Honoré, Bent; Christensen, Lars Porskjær; Hansen, Paul Robert; Franzyk, Henrik; Mirza, Osman; Prabhala, Bala Krishna.
In: Biochemical and Biophysical Research Communications, Vol. 661, 2023, p. 42-49.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Physiological response in E. coli to YdgR overexpression depends on whether the protein has an intact function
AU - Sajid, Salvia
AU - Salas, Lilia Hernandez
AU - Rafiq, Maria
AU - Lund, Torben
AU - Jørgensen, Mikkel Girke
AU - Honoré, Bent
AU - Christensen, Lars Porskjær
AU - Hansen, Paul Robert
AU - Franzyk, Henrik
AU - Mirza, Osman
AU - Prabhala, Bala Krishna
N1 - Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Membrane transport proteins are essential for the transport of a wide variety of molecules across the cell membrane to maintain cellular homeostasis. Generally, these transport proteins can be overexpressed in a suitable host (bacteria, yeast, or mammalian cells), and it is well documented that overexpression of membrane proteins alters the global metabolomic and proteomic profiles of the host cells. In the present study, we investigated the physiological consequences of overexpression of a membrane transport protein YdgR that belongs to the POT/PTR family from E. coli by using the lab strain BL21 (DE3)pLysS in its functional and attenuated mutant YdgR-E33Q. We found significant differences between the omics (metabolomics and proteomics) profiles of the cells expressing functional YdgR as compared to cells expressing attenuated YdgR, e.g., upregulation of several uncharacterized y-proteins and enzymes involved in the metabolism of peptides and amino acids. Furthermore, molecular network analysis suggested a relatively higher presence of proline-containing tripeptides in cells expressing functional YdgR. We envisage that an in-depth investigation of physiological alterations due to protein over-expression may be used for the deorphanization of the y-gene transportome.
AB - Membrane transport proteins are essential for the transport of a wide variety of molecules across the cell membrane to maintain cellular homeostasis. Generally, these transport proteins can be overexpressed in a suitable host (bacteria, yeast, or mammalian cells), and it is well documented that overexpression of membrane proteins alters the global metabolomic and proteomic profiles of the host cells. In the present study, we investigated the physiological consequences of overexpression of a membrane transport protein YdgR that belongs to the POT/PTR family from E. coli by using the lab strain BL21 (DE3)pLysS in its functional and attenuated mutant YdgR-E33Q. We found significant differences between the omics (metabolomics and proteomics) profiles of the cells expressing functional YdgR as compared to cells expressing attenuated YdgR, e.g., upregulation of several uncharacterized y-proteins and enzymes involved in the metabolism of peptides and amino acids. Furthermore, molecular network analysis suggested a relatively higher presence of proline-containing tripeptides in cells expressing functional YdgR. We envisage that an in-depth investigation of physiological alterations due to protein over-expression may be used for the deorphanization of the y-gene transportome.
U2 - 10.1016/j.bbrc.2023.04.032
DO - 10.1016/j.bbrc.2023.04.032
M3 - Journal article
C2 - 37087797
VL - 661
SP - 42
EP - 49
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
ER -
ID: 344715729