Povidone iodine treatment is deleterious to human ocular surface conjunctival cells in culture
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Povidone iodine treatment is deleterious to human ocular surface conjunctival cells in culture. / Swift, William; Bair, Jeffrey A; Chen, Wanxue; Li, Michael; Lie, Sole; Li, Dayu; Yang, Menglu; Shatos, Marie A; Hodges, Robin R; Kolko, Miriam; Utheim, Tor P; Scott, Wendell; Dartt, Darlene A.
In: BMJ Open Ophthalmology, Vol. 5, No. 1, e000545, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Povidone iodine treatment is deleterious to human ocular surface conjunctival cells in culture
AU - Swift, William
AU - Bair, Jeffrey A
AU - Chen, Wanxue
AU - Li, Michael
AU - Lie, Sole
AU - Li, Dayu
AU - Yang, Menglu
AU - Shatos, Marie A
AU - Hodges, Robin R
AU - Kolko, Miriam
AU - Utheim, Tor P
AU - Scott, Wendell
AU - Dartt, Darlene A
N1 - © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
PY - 2020
Y1 - 2020
N2 - Objective: To determine the effect of povidone iodine (PI), an antiseptic commonly used prior to ocular surgery, on viability of mixed populations of conjunctival stratified squamous and goblet cells, purified conjunctival goblet cells and purified conjunctival stromal fibroblasts in primary culture.Methods and analysis: Mixed population of epithelial cells (stratified squamous and goblet cells), goblet cells and fibroblasts were grown in culture from pieces of human conjunctiva using either supplemented DMEM/F12 or RPMI. Cell type was evaluated by immunofluorescence microscopy. Cells were treated for 5 min with phosphate-buffered saline (PBS); 0.25%, 2.5%, 5% or 10% PI in PBS; or a positive control of 30% H2O2. Cell viability was determined using Alamar Blue fluorescence and a live/dead kit using calcein/AM and ethidium homodimer-1 (EH-1).Results: Mixed populations of epithelial cells, goblet cells and fibroblasts were characterised by immunofluorescence microscopy. As determined with Alamar Blue fluorescence, all concentrations of PI significantly decreased the number of cells from all three preparation types compared with PBS. As determined by calcein/EH-1 viability test, mixed populations of cells and fibroblasts were less sensitive to PI treatment than goblet cells. All concentrations of PI, except for 0.25% used with goblet cells, substantially increased the number of dead cells for all cell populations. The H2O2 control also significantly decreased the number and viability of all three types of cells in both tests.Conclusion: We conclude that PI, which is commonly used prior to ocular surgeries, is detrimental to human conjunctival stratified squamous cells, goblet cells and fibroblasts in culture.
AB - Objective: To determine the effect of povidone iodine (PI), an antiseptic commonly used prior to ocular surgery, on viability of mixed populations of conjunctival stratified squamous and goblet cells, purified conjunctival goblet cells and purified conjunctival stromal fibroblasts in primary culture.Methods and analysis: Mixed population of epithelial cells (stratified squamous and goblet cells), goblet cells and fibroblasts were grown in culture from pieces of human conjunctiva using either supplemented DMEM/F12 or RPMI. Cell type was evaluated by immunofluorescence microscopy. Cells were treated for 5 min with phosphate-buffered saline (PBS); 0.25%, 2.5%, 5% or 10% PI in PBS; or a positive control of 30% H2O2. Cell viability was determined using Alamar Blue fluorescence and a live/dead kit using calcein/AM and ethidium homodimer-1 (EH-1).Results: Mixed populations of epithelial cells, goblet cells and fibroblasts were characterised by immunofluorescence microscopy. As determined with Alamar Blue fluorescence, all concentrations of PI significantly decreased the number of cells from all three preparation types compared with PBS. As determined by calcein/EH-1 viability test, mixed populations of cells and fibroblasts were less sensitive to PI treatment than goblet cells. All concentrations of PI, except for 0.25% used with goblet cells, substantially increased the number of dead cells for all cell populations. The H2O2 control also significantly decreased the number and viability of all three types of cells in both tests.Conclusion: We conclude that PI, which is commonly used prior to ocular surgeries, is detrimental to human conjunctival stratified squamous cells, goblet cells and fibroblasts in culture.
U2 - 10.1136/bmjophth-2020-000545
DO - 10.1136/bmjophth-2020-000545
M3 - Journal article
C2 - 32995498
VL - 5
JO - BMJ Open Ophthalmology
JF - BMJ Open Ophthalmology
SN - 2397-3269
IS - 1
M1 - e000545
ER -
ID: 256210416