Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size. / Wadhwa, Abishek; Bobak, Thomas R.; Bohrmann, Lennart; Geczy, Reka; Sekar, Sathiya; Sathyanarayanan, Gowtham; Kutter, Jörg P.; Franzyk, Henrik; Foged, Camilla; Saatchi, Katayoun; Häfeli, Urs O.

In: OpenNano, Vol. 13, 100180, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wadhwa, A, Bobak, TR, Bohrmann, L, Geczy, R, Sekar, S, Sathyanarayanan, G, Kutter, JP, Franzyk, H, Foged, C, Saatchi, K & Häfeli, UO 2023, 'Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size', OpenNano, vol. 13, 100180. https://doi.org/10.1016/j.onano.2023.100180

APA

Wadhwa, A., Bobak, T. R., Bohrmann, L., Geczy, R., Sekar, S., Sathyanarayanan, G., Kutter, J. P., Franzyk, H., Foged, C., Saatchi, K., & Häfeli, U. O. (2023). Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size. OpenNano, 13, [100180]. https://doi.org/10.1016/j.onano.2023.100180

Vancouver

Wadhwa A, Bobak TR, Bohrmann L, Geczy R, Sekar S, Sathyanarayanan G et al. Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size. OpenNano. 2023;13. 100180. https://doi.org/10.1016/j.onano.2023.100180

Author

Wadhwa, Abishek ; Bobak, Thomas R. ; Bohrmann, Lennart ; Geczy, Reka ; Sekar, Sathiya ; Sathyanarayanan, Gowtham ; Kutter, Jörg P. ; Franzyk, Henrik ; Foged, Camilla ; Saatchi, Katayoun ; Häfeli, Urs O. / Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size. In: OpenNano. 2023 ; Vol. 13.

Bibtex

@article{80b8d9ae96344c48bbf9583de1126317,
title = "Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size",
abstract = "Nanomedicines based on nanoparticles rely both on the potency of the drug as well as the efficiency of the delivery system, for which particle size plays a crucial role. For the intracellular delivery of small interference RNA (siRNA), lipid-polymer nanoparticle (LPN) hybrid systems constitute a safe and highly effective class of delivery systems. In the present study, we employ a microfluidics method for the manufacturing of spherical siRNA-loaded LPNs for pulmonary delivery with distinct size distributions with average diameters of approximately 70, 110, and 220 nm. We designed an optically clear, inexpensive thiol-ene polymeric microfluidic chip prototype that is compatible with standard {\textquoteleft}soft-lithography{\textquoteright} techniques, allows for replica molding, and is resistant to harsh solvents. By using SPECT/CT in vivo imaging, we show comparable pulmonary clearance patterns of all three differently sized LPN formulations following intratracheal administration. Also, negligible accumulation in the liver was observed.",
author = "Abishek Wadhwa and Bobak, {Thomas R.} and Lennart Bohrmann and Reka Geczy and Sathiya Sekar and Gowtham Sathyanarayanan and Kutter, {J{\"o}rg P.} and Henrik Franzyk and Camilla Foged and Katayoun Saatchi and H{\"a}feli, {Urs O.}",
year = "2023",
doi = "10.1016/j.onano.2023.100180",
language = "English",
volume = "13",
journal = "OpenNano",
issn = "2352-9520",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Pulmonary delivery of siRNA-loaded lipid-polymer hybrid nanoparticles: Effect of nanoparticle size

AU - Wadhwa, Abishek

AU - Bobak, Thomas R.

AU - Bohrmann, Lennart

AU - Geczy, Reka

AU - Sekar, Sathiya

AU - Sathyanarayanan, Gowtham

AU - Kutter, Jörg P.

AU - Franzyk, Henrik

AU - Foged, Camilla

AU - Saatchi, Katayoun

AU - Häfeli, Urs O.

PY - 2023

Y1 - 2023

N2 - Nanomedicines based on nanoparticles rely both on the potency of the drug as well as the efficiency of the delivery system, for which particle size plays a crucial role. For the intracellular delivery of small interference RNA (siRNA), lipid-polymer nanoparticle (LPN) hybrid systems constitute a safe and highly effective class of delivery systems. In the present study, we employ a microfluidics method for the manufacturing of spherical siRNA-loaded LPNs for pulmonary delivery with distinct size distributions with average diameters of approximately 70, 110, and 220 nm. We designed an optically clear, inexpensive thiol-ene polymeric microfluidic chip prototype that is compatible with standard ‘soft-lithography’ techniques, allows for replica molding, and is resistant to harsh solvents. By using SPECT/CT in vivo imaging, we show comparable pulmonary clearance patterns of all three differently sized LPN formulations following intratracheal administration. Also, negligible accumulation in the liver was observed.

AB - Nanomedicines based on nanoparticles rely both on the potency of the drug as well as the efficiency of the delivery system, for which particle size plays a crucial role. For the intracellular delivery of small interference RNA (siRNA), lipid-polymer nanoparticle (LPN) hybrid systems constitute a safe and highly effective class of delivery systems. In the present study, we employ a microfluidics method for the manufacturing of spherical siRNA-loaded LPNs for pulmonary delivery with distinct size distributions with average diameters of approximately 70, 110, and 220 nm. We designed an optically clear, inexpensive thiol-ene polymeric microfluidic chip prototype that is compatible with standard ‘soft-lithography’ techniques, allows for replica molding, and is resistant to harsh solvents. By using SPECT/CT in vivo imaging, we show comparable pulmonary clearance patterns of all three differently sized LPN formulations following intratracheal administration. Also, negligible accumulation in the liver was observed.

U2 - 10.1016/j.onano.2023.100180

DO - 10.1016/j.onano.2023.100180

M3 - Journal article

VL - 13

JO - OpenNano

JF - OpenNano

SN - 2352-9520

M1 - 100180

ER -

ID: 361385126