Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo. / McNair, Laura M.; Mason, Graeme F.; Chowdhury, Golam M.I.; Jiang, Lihong; Ma, Xiaoxian; Rothman, Douglas L.; Waagepetersen, Helle S.; Behar, Kevin L.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 42, No. 8, 2022, p. 1507–1523.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

McNair, LM, Mason, GF, Chowdhury, GMI, Jiang, L, Ma, X, Rothman, DL, Waagepetersen, HS & Behar, KL 2022, 'Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo', Journal of Cerebral Blood Flow and Metabolism, vol. 42, no. 8, pp. 1507–1523. https://doi.org/10.1177/0271678X221074211

APA

McNair, L. M., Mason, G. F., Chowdhury, G. M. I., Jiang, L., Ma, X., Rothman, D. L., Waagepetersen, H. S., & Behar, K. L. (2022). Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo. Journal of Cerebral Blood Flow and Metabolism, 42(8), 1507–1523. https://doi.org/10.1177/0271678X221074211

Vancouver

McNair LM, Mason GF, Chowdhury GMI, Jiang L, Ma X, Rothman DL et al. Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo. Journal of Cerebral Blood Flow and Metabolism. 2022;42(8):1507–1523. https://doi.org/10.1177/0271678X221074211

Author

McNair, Laura M. ; Mason, Graeme F. ; Chowdhury, Golam M.I. ; Jiang, Lihong ; Ma, Xiaoxian ; Rothman, Douglas L. ; Waagepetersen, Helle S. ; Behar, Kevin L. / Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo. In: Journal of Cerebral Blood Flow and Metabolism. 2022 ; Vol. 42, No. 8. pp. 1507–1523.

Bibtex

@article{b3b45b4dad3d4e86a528c3d1f1ad7d6c,
title = "Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo",
abstract = "Anaplerosis occurs predominately in astroglia through the action of pyruvate carboxylase (PC). The rate of PC (Vpc) has been reported for cerebral cortex (or whole brain) of awake humans and anesthetized rodents, but regional brain rates remain largely unknown and, hence, were subjected to investigation in the current study. Awake male rats were infused with either [2-13C]glucose or [1-13C]glucose (n = 27/30) for 8, 15, 30, 60 or 120 min, followed by rapid euthanasia with focused-beam microwave irradiation to the brain. Blood plasma and extracts of cerebellum, hippocampus, striatum, and cerebral cortex were analyzed by 1H-[13C]-NMR to establish 13C-enrichment time courses for glutamate-C4,C3,C2, glutamine-C4,C3, GABA-C2,C3,C4 and aspartate-C2,C3. Metabolic rates were determined by fitting a three-compartment metabolic model (glutamatergic and GABAergic neurons and astroglia) to the eighteen time courses. Vpc varied by 44% across brain regions, being lowest in the cerebellum (0.087 ± 0.004 µmol/g/min) and highest in striatum (0.125 ± 0.009) with intermediate values in cerebral cortex (0.106 ± 0.005) and hippocampus (0.114 ± 0.005). Vpc constituted 13–19% of the oxidative glucose consumption rate. Combination of cerebral cortical data with literature values revealed a positive correlation between Vpc and the rates of glutamate/glutamine-cycling and oxidative glucose consumption, respectively, consistent with earlier observations.",
keywords = "Anaplerosis, cerebellum, cerebral cortex, hippocampus, striatum",
author = "McNair, {Laura M.} and Mason, {Graeme F.} and Chowdhury, {Golam M.I.} and Lihong Jiang and Xiaoxian Ma and Rothman, {Douglas L.} and Waagepetersen, {Helle S.} and Behar, {Kevin L.}",
note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: LMM acknowledges the Department of Drug Design and Pharmacology (UCPH, Denmark) for granting of a PhD scholarship. This work was supported in part by NIH NIMH grants MH095104 and MH109159, NIH NIDDK grant R01 DK108283, NIH NIAAA grant R01 AA021984 and R21 AA028628, and a grant from Aase og Ejnar Danielsens Fond. Acknowledgements Publisher Copyright: {\textcopyright} The Author(s) 2022.",
year = "2022",
doi = "10.1177/0271678X221074211",
language = "English",
volume = "42",
pages = "1507–1523",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "SAGE Publications",
number = "8",

}

RIS

TY - JOUR

T1 - Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo

AU - McNair, Laura M.

AU - Mason, Graeme F.

AU - Chowdhury, Golam M.I.

AU - Jiang, Lihong

AU - Ma, Xiaoxian

AU - Rothman, Douglas L.

AU - Waagepetersen, Helle S.

AU - Behar, Kevin L.

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: LMM acknowledges the Department of Drug Design and Pharmacology (UCPH, Denmark) for granting of a PhD scholarship. This work was supported in part by NIH NIMH grants MH095104 and MH109159, NIH NIDDK grant R01 DK108283, NIH NIAAA grant R01 AA021984 and R21 AA028628, and a grant from Aase og Ejnar Danielsens Fond. Acknowledgements Publisher Copyright: © The Author(s) 2022.

PY - 2022

Y1 - 2022

N2 - Anaplerosis occurs predominately in astroglia through the action of pyruvate carboxylase (PC). The rate of PC (Vpc) has been reported for cerebral cortex (or whole brain) of awake humans and anesthetized rodents, but regional brain rates remain largely unknown and, hence, were subjected to investigation in the current study. Awake male rats were infused with either [2-13C]glucose or [1-13C]glucose (n = 27/30) for 8, 15, 30, 60 or 120 min, followed by rapid euthanasia with focused-beam microwave irradiation to the brain. Blood plasma and extracts of cerebellum, hippocampus, striatum, and cerebral cortex were analyzed by 1H-[13C]-NMR to establish 13C-enrichment time courses for glutamate-C4,C3,C2, glutamine-C4,C3, GABA-C2,C3,C4 and aspartate-C2,C3. Metabolic rates were determined by fitting a three-compartment metabolic model (glutamatergic and GABAergic neurons and astroglia) to the eighteen time courses. Vpc varied by 44% across brain regions, being lowest in the cerebellum (0.087 ± 0.004 µmol/g/min) and highest in striatum (0.125 ± 0.009) with intermediate values in cerebral cortex (0.106 ± 0.005) and hippocampus (0.114 ± 0.005). Vpc constituted 13–19% of the oxidative glucose consumption rate. Combination of cerebral cortical data with literature values revealed a positive correlation between Vpc and the rates of glutamate/glutamine-cycling and oxidative glucose consumption, respectively, consistent with earlier observations.

AB - Anaplerosis occurs predominately in astroglia through the action of pyruvate carboxylase (PC). The rate of PC (Vpc) has been reported for cerebral cortex (or whole brain) of awake humans and anesthetized rodents, but regional brain rates remain largely unknown and, hence, were subjected to investigation in the current study. Awake male rats were infused with either [2-13C]glucose or [1-13C]glucose (n = 27/30) for 8, 15, 30, 60 or 120 min, followed by rapid euthanasia with focused-beam microwave irradiation to the brain. Blood plasma and extracts of cerebellum, hippocampus, striatum, and cerebral cortex were analyzed by 1H-[13C]-NMR to establish 13C-enrichment time courses for glutamate-C4,C3,C2, glutamine-C4,C3, GABA-C2,C3,C4 and aspartate-C2,C3. Metabolic rates were determined by fitting a three-compartment metabolic model (glutamatergic and GABAergic neurons and astroglia) to the eighteen time courses. Vpc varied by 44% across brain regions, being lowest in the cerebellum (0.087 ± 0.004 µmol/g/min) and highest in striatum (0.125 ± 0.009) with intermediate values in cerebral cortex (0.106 ± 0.005) and hippocampus (0.114 ± 0.005). Vpc constituted 13–19% of the oxidative glucose consumption rate. Combination of cerebral cortical data with literature values revealed a positive correlation between Vpc and the rates of glutamate/glutamine-cycling and oxidative glucose consumption, respectively, consistent with earlier observations.

KW - Anaplerosis

KW - cerebellum

KW - cerebral cortex

KW - hippocampus

KW - striatum

U2 - 10.1177/0271678X221074211

DO - 10.1177/0271678X221074211

M3 - Journal article

C2 - 35048735

AN - SCOPUS:85123478846

VL - 42

SP - 1507

EP - 1523

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 8

ER -

ID: 291671955