Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra

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Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra. / Thomsen, S.; Hansen, Harald S.; Nyman, U.

In: Planta Medica, Vol. 57, No. 3, 01.01.1991, p. 232-236.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Thomsen, S, Hansen, HS & Nyman, U 1991, 'Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra', Planta Medica, vol. 57, no. 3, pp. 232-236.

APA

Thomsen, S., Hansen, H. S., & Nyman, U. (1991). Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra. Planta Medica, 57(3), 232-236.

Vancouver

Thomsen S, Hansen HS, Nyman U. Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra. Planta Medica. 1991 Jan 1;57(3):232-236.

Author

Thomsen, S. ; Hansen, Harald S. ; Nyman, U. / Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra. In: Planta Medica. 1991 ; Vol. 57, No. 3. pp. 232-236.

Bibtex

@article{8d38b456e11c4d20967f10a3a1ffd36f,
title = "Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra",
abstract = "Phytolacca dodecandra (L'Herit) grown in cell cultures was investigated for content of ribosome-inhibiting proteins, which was evaluated hy measuring inhibition of protein synthesis in a cell-free rat liver extract. Calli initiated from leaf, cotyledon, radicle, and hypocotyl and suspension cells initiated from leaf and cotyledon exhibited protein synthesis-inhibiting activity. Ribosome-inhibiting proteins were purified at least 14 times from suspension cells of P. dodecandra. The purified protein fraction contained two proteins as seen by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The relative molecular masses were 30,000 and 31,000 and they showed a pI > 9.3. These new RIP's were shown to be different from dodecandrin with respect to molecular mass.",
author = "S. Thomsen and Hansen, {Harald S.} and U. Nyman",
year = "1991",
month = jan,
day = "1",
language = "English",
volume = "57",
pages = "232--236",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "GeorgThieme Verlag",
number = "3",

}

RIS

TY - JOUR

T1 - Ribosome-inhibiting proteins from in vitro cultures of Phytolacca dodecandra

AU - Thomsen, S.

AU - Hansen, Harald S.

AU - Nyman, U.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Phytolacca dodecandra (L'Herit) grown in cell cultures was investigated for content of ribosome-inhibiting proteins, which was evaluated hy measuring inhibition of protein synthesis in a cell-free rat liver extract. Calli initiated from leaf, cotyledon, radicle, and hypocotyl and suspension cells initiated from leaf and cotyledon exhibited protein synthesis-inhibiting activity. Ribosome-inhibiting proteins were purified at least 14 times from suspension cells of P. dodecandra. The purified protein fraction contained two proteins as seen by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The relative molecular masses were 30,000 and 31,000 and they showed a pI > 9.3. These new RIP's were shown to be different from dodecandrin with respect to molecular mass.

AB - Phytolacca dodecandra (L'Herit) grown in cell cultures was investigated for content of ribosome-inhibiting proteins, which was evaluated hy measuring inhibition of protein synthesis in a cell-free rat liver extract. Calli initiated from leaf, cotyledon, radicle, and hypocotyl and suspension cells initiated from leaf and cotyledon exhibited protein synthesis-inhibiting activity. Ribosome-inhibiting proteins were purified at least 14 times from suspension cells of P. dodecandra. The purified protein fraction contained two proteins as seen by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The relative molecular masses were 30,000 and 31,000 and they showed a pI > 9.3. These new RIP's were shown to be different from dodecandrin with respect to molecular mass.

UR - http://www.scopus.com/inward/record.url?scp=0025909306&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:0025909306

VL - 57

SP - 232

EP - 236

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 3

ER -

ID: 45561672