Role of pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion
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Role of pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion. / Kristiansen, Sarah Brøgger; Skovsted, Gry Freja; Berchtold, Lukas Adrian; Radziwon-Balicka, Aneta; Dreisig, Karin; Edvinsson, Lars; Sheykhzade, Majid; Haanes, Kristian Agmund.
In: Scandinavian Cardiovascular Journal, Vol. 52, No. 6, 2018, p. 340-343.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Role of pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion
AU - Kristiansen, Sarah Brøgger
AU - Skovsted, Gry Freja
AU - Berchtold, Lukas Adrian
AU - Radziwon-Balicka, Aneta
AU - Dreisig, Karin
AU - Edvinsson, Lars
AU - Sheykhzade, Majid
AU - Haanes, Kristian Agmund
PY - 2018
Y1 - 2018
N2 - OBJECTIVES: The purinergic system has not been investigated in detail following ischemia/reperfusion (I/R) injury in the heart. In the present study we focus on both release and response to extracellular adenosine triphosphate (ATP). Pannexin (Panx) channels have been shown to be involved in ATP release from myocytes and can activate P2X1 and P2Y2 receptors on the coronary artery.DESIGN: We applied a well characterized I/R model in rats, with 24 hours of reperfusion. Panx expression in the myocardial tissue was measured with quantitative polymerase chain reaction (qPCR) and flow cytometry. ATP release was detected in situ using luminescence and the vascular response to nucleotides determined in a wire myograph.RESULTS: Here we show that Panx expression is increased after experimental myocardial I/R, leading to an increase in extracellular ATP release, which could be inhibited by probenecid. Functional studies revealed that the P2Y2 receptor dependent contraction is reduced in the coronary artery after I/R, which might be a response to the increased ATP levels.CONCLUSION: We therefore, conclude that the regulation of the arterial purinergic system minimizes coronary contractions following ischemia.
AB - OBJECTIVES: The purinergic system has not been investigated in detail following ischemia/reperfusion (I/R) injury in the heart. In the present study we focus on both release and response to extracellular adenosine triphosphate (ATP). Pannexin (Panx) channels have been shown to be involved in ATP release from myocytes and can activate P2X1 and P2Y2 receptors on the coronary artery.DESIGN: We applied a well characterized I/R model in rats, with 24 hours of reperfusion. Panx expression in the myocardial tissue was measured with quantitative polymerase chain reaction (qPCR) and flow cytometry. ATP release was detected in situ using luminescence and the vascular response to nucleotides determined in a wire myograph.RESULTS: Here we show that Panx expression is increased after experimental myocardial I/R, leading to an increase in extracellular ATP release, which could be inhibited by probenecid. Functional studies revealed that the P2Y2 receptor dependent contraction is reduced in the coronary artery after I/R, which might be a response to the increased ATP levels.CONCLUSION: We therefore, conclude that the regulation of the arterial purinergic system minimizes coronary contractions following ischemia.
U2 - 10.1080/14017431.2018.1552793
DO - 10.1080/14017431.2018.1552793
M3 - Journal article
C2 - 30481075
VL - 52
SP - 340
EP - 343
JO - Scandinavian Cardiovascular Journal
JF - Scandinavian Cardiovascular Journal
SN - 1401-7458
IS - 6
ER -
ID: 209624646