Synthesis of the phytohormone [11C]methyl jasmonate via methylation on a C18 Sep Pak™ cartridge
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Synthesis of the phytohormone [11C]methyl jasmonate via methylation on a C18 Sep Pak™ cartridge. / Herth, Matthias M.; Thorpe, Michael R.; Ferrieri, Richard A.
In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 48, No. 5, 01.04.2005, p. 379-386.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Synthesis of the phytohormone [11C]methyl jasmonate via methylation on a C18 Sep Pak™ cartridge
AU - Herth, Matthias M.
AU - Thorpe, Michael R.
AU - Ferrieri, Richard A.
PY - 2005/4/1
Y1 - 2005/4/1
N2 - [11C]labeled (±)-methyl jasmonate was synthesized using a C18 Sep Pak™ at ∼100°C to sustain a solid-supported 11C-methylation reaction of sodium (±)-jasmonate using [ 11C]methyl iodide. After reaction, the Sep Pak was rinsed with acetone to elute the labeled product, and the solvent evaporated rendering [11C]-(+)-methyl jasmonate at 96% radiochemical purity. The substrate, (±)-jasmonic acid, was retained on the Sep Pak so further chromatography was unnecessary. Total synthesis time was 25 min from the end of bombardment (EOB) which included 15 min to generate [11C]methyl iodide using the GE Medical Systems PET Trace MeI system, 5 min for reaction and extraction from the cartridge, and 5 min to reformulate the product for plant administration. An overall radiochemical yield (at EOB) of 17 ± 4.3% was obtained by this process, typically producing 10mCi of purified radiotracer. A specific activity of 0.5 Ci/μmol was achieved using a short 3 min cyclotron beam to produce the starting 11C.
AB - [11C]labeled (±)-methyl jasmonate was synthesized using a C18 Sep Pak™ at ∼100°C to sustain a solid-supported 11C-methylation reaction of sodium (±)-jasmonate using [ 11C]methyl iodide. After reaction, the Sep Pak was rinsed with acetone to elute the labeled product, and the solvent evaporated rendering [11C]-(+)-methyl jasmonate at 96% radiochemical purity. The substrate, (±)-jasmonic acid, was retained on the Sep Pak so further chromatography was unnecessary. Total synthesis time was 25 min from the end of bombardment (EOB) which included 15 min to generate [11C]methyl iodide using the GE Medical Systems PET Trace MeI system, 5 min for reaction and extraction from the cartridge, and 5 min to reformulate the product for plant administration. An overall radiochemical yield (at EOB) of 17 ± 4.3% was obtained by this process, typically producing 10mCi of purified radiotracer. A specific activity of 0.5 Ci/μmol was achieved using a short 3 min cyclotron beam to produce the starting 11C.
KW - C-labeled phytohormone
KW - [C]methyl jasmonate
KW - Solid-supported C-methylation
UR - http://www.scopus.com/inward/record.url?scp=17844398236&partnerID=8YFLogxK
U2 - 10.1002/jlcr.933
DO - 10.1002/jlcr.933
M3 - Journal article
AN - SCOPUS:17844398236
VL - 48
SP - 379
EP - 386
JO - Journal of Labelled Compounds and Radiopharmaceuticals
JF - Journal of Labelled Compounds and Radiopharmaceuticals
SN - 0362-4803
IS - 5
ER -
ID: 199384822