The labeling of unsaturated γ-hydroxybutyric acid by heavy isotopes of hydrogen: iridium complex-mediated H/D exchange by C─H bond activation vs reduction by boro-deuterides/tritides
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The labeling of unsaturated γ-hydroxybutyric acid by heavy isotopes of hydrogen : iridium complex-mediated H/D exchange by C─H bond activation vs reduction by boro-deuterides/tritides. / Marek, Aleš; Pedersen, Martin H F; Vogensen, Stine Byskov; Clausen, Rasmus P; Frølund, Bente; Elbert, Tomáš.
In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 59, No. 12, 10.2016, p. 476-483.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The labeling of unsaturated γ-hydroxybutyric acid by heavy isotopes of hydrogen
T2 - iridium complex-mediated H/D exchange by C─H bond activation vs reduction by boro-deuterides/tritides
AU - Marek, Aleš
AU - Pedersen, Martin H F
AU - Vogensen, Stine Byskov
AU - Clausen, Rasmus P
AU - Frølund, Bente
AU - Elbert, Tomáš
N1 - Copyright © 2016 John Wiley & Sons, Ltd.
PY - 2016/10
Y1 - 2016/10
N2 - 3-Hydroxycyclopent-1-ene-1-carboxylic acid (HOCPCA (1)) is a potent ligand for high-affinity γ-hydroxybutyric acid binding sites in the central nervous system. Various approaches to the introduction of a hydrogen label onto the HOCPCA skeleton are reported. The outcomes of the feasible C─H activation of olefin carbon (C-2) by iridium catalyst are compared with the reduction of the carbonyl group (C-3) by freshly prepared borodeuterides. The most efficient iridium catalysts proved to be Kerr bulky phosphine N-heterocyclic species providing outstanding deuterium enrichment (up to 91%) in a short period of time. The highest deuterium enrichment (>99%) was achieved through the reduction of ketone precursor 2 by lithium trimethoxyborodeuteride. Hence, analogical conditions were used for the tritiation experiment. [(3) H]-HOCPCA selectively labeled on the position C-3 was synthetized with radiochemical purity >99%, an isolated yield of 637 mCi and specific activity = 28.9 Ci/mmol.
AB - 3-Hydroxycyclopent-1-ene-1-carboxylic acid (HOCPCA (1)) is a potent ligand for high-affinity γ-hydroxybutyric acid binding sites in the central nervous system. Various approaches to the introduction of a hydrogen label onto the HOCPCA skeleton are reported. The outcomes of the feasible C─H activation of olefin carbon (C-2) by iridium catalyst are compared with the reduction of the carbonyl group (C-3) by freshly prepared borodeuterides. The most efficient iridium catalysts proved to be Kerr bulky phosphine N-heterocyclic species providing outstanding deuterium enrichment (up to 91%) in a short period of time. The highest deuterium enrichment (>99%) was achieved through the reduction of ketone precursor 2 by lithium trimethoxyborodeuteride. Hence, analogical conditions were used for the tritiation experiment. [(3) H]-HOCPCA selectively labeled on the position C-3 was synthetized with radiochemical purity >99%, an isolated yield of 637 mCi and specific activity = 28.9 Ci/mmol.
U2 - 10.1002/jlcr.3432
DO - 10.1002/jlcr.3432
M3 - Journal article
C2 - 27593893
VL - 59
SP - 476
EP - 483
JO - Journal of Labelled Compounds and Radiopharmaceuticals
JF - Journal of Labelled Compounds and Radiopharmaceuticals
SN - 0362-4803
IS - 12
ER -
ID: 168285655