The potential of the essential fatty acid-deficient hairless rat as a psoriasis screening model for topical anti-proliferative drugs
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The potential of the essential fatty acid-deficient hairless rat as a psoriasis screening model for topical anti-proliferative drugs. / Jensen, M.; Groth, L.; Fullerton, A.; Hølmer, Gunhild Kofoed; Hansen, Harald S.
In: Skin pharmacology and applied skin physiology, Vol. 15, No. 6, 01.01.2002, p. 401-413.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The potential of the essential fatty acid-deficient hairless rat as a psoriasis screening model for topical anti-proliferative drugs
AU - Jensen, M.
AU - Groth, L.
AU - Fullerton, A.
AU - Hølmer, Gunhild Kofoed
AU - Hansen, Harald S.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - The objective of this study was to establish essential fatty acid deficiency (EFAD) in hairless rats and investigate the potential of this model as a psoriasis screening model by testing the effects of calcipotriol and dithranol on differentiation and proliferation in the epidermis. Hairless rats were fed with a fat-free diet lacking linoleic acid. The EFAD condition was established within 8 weeks. In order to ensure that this condition had been established, several parameters were measured and observed, i.e. animal weight, water consumption, transepidermal water loss, clinical skin symptoms, histology of the epidermis and fatty acid analysis of serum and skin. Immediately after the EFAD condition had been established, the animals were treated with dithranol ointment or different concentrations of calcipotriol solution. A reduction in epidermal thickness of 15-20% was seen after the treatment with calcipotriol. Dithranol and its coal tar-containing vehicle also showed a reductive effect on epidermal thickness. EFAD hairless rats possess various histological changes resembling psoriasis. These histological changes normalise during treatment with anti-psoriatic drugs as calcipotriol, dithranol and coal tar. The results of the present study indicate that the EFAD rat may be a useful model for studies of anti-psoriatic drugs affecting cell proliferation.
AB - The objective of this study was to establish essential fatty acid deficiency (EFAD) in hairless rats and investigate the potential of this model as a psoriasis screening model by testing the effects of calcipotriol and dithranol on differentiation and proliferation in the epidermis. Hairless rats were fed with a fat-free diet lacking linoleic acid. The EFAD condition was established within 8 weeks. In order to ensure that this condition had been established, several parameters were measured and observed, i.e. animal weight, water consumption, transepidermal water loss, clinical skin symptoms, histology of the epidermis and fatty acid analysis of serum and skin. Immediately after the EFAD condition had been established, the animals were treated with dithranol ointment or different concentrations of calcipotriol solution. A reduction in epidermal thickness of 15-20% was seen after the treatment with calcipotriol. Dithranol and its coal tar-containing vehicle also showed a reductive effect on epidermal thickness. EFAD hairless rats possess various histological changes resembling psoriasis. These histological changes normalise during treatment with anti-psoriatic drugs as calcipotriol, dithranol and coal tar. The results of the present study indicate that the EFAD rat may be a useful model for studies of anti-psoriatic drugs affecting cell proliferation.
UR - http://www.scopus.com/inward/record.url?scp=0036906876&partnerID=8YFLogxK
U2 - 10.1159/000066453
DO - 10.1159/000066453
M3 - Journal article
AN - SCOPUS:0036906876
VL - 15
SP - 401
EP - 413
JO - Skin Pharmacology and Applied Skin Physiology
JF - Skin Pharmacology and Applied Skin Physiology
SN - 1422-2868
IS - 6
ER -
ID: 45561711