The repertoire of trace amine G-protein-coupled receptors: large expansion in zebrafish
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The repertoire of trace amine G-protein-coupled receptors : large expansion in zebrafish. / Gloriam, David E.; Bjarnadóttir, Thóra K; Yan, Yi-Lin; Postlethwait, John H; Schiöth, Helgi B; Fredriksson, Robert.
In: Molecular Phylogenetics and Evolution, Vol. 35, No. 2, 05.2005, p. 470-82.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The repertoire of trace amine G-protein-coupled receptors
T2 - large expansion in zebrafish
AU - Gloriam, David E.
AU - Bjarnadóttir, Thóra K
AU - Yan, Yi-Lin
AU - Postlethwait, John H
AU - Schiöth, Helgi B
AU - Fredriksson, Robert
PY - 2005/5
Y1 - 2005/5
N2 - Trace amines, such as tyramine, beta-phenylethylamine, tryptamine, and octopamine, are present in trace levels in nervous systems and bind a specific family of G-protein-coupled receptors (GPCR), but the function or origin of this system is not well understood. We searched the genomes of several eukaryotic species for receptors similar to the mammalian trace amine (TA) receptor subfamily. We identified 18 new receptors in rodents that are orthologous to the previously known TA-receptors. Remarkably, we found 57 receptors (and 40 pseudogenes) of this type in the zebrafish (Danio rerio), while fugu (Takifugu rubripes) had only eight receptors (and seven pseudogenes). We mapped 47 of the zebrafish TA-receptors on chromosomes using radiation hybrid panels and meiotic mapping. The results, together with the degree of conservation and phylogenetic relationships displayed among the zebrafish receptors suggest that the family arose through several different mechanisms involving tetraploidization, block duplications, and local duplication events. Interestingly, these vertebrate TA-receptors do not show a close evolutionary relationship to the invertebrate TA-binding receptors in fruitfly (Drosophila melanogaster), indicating that the ability to bind TA have evolved at least twice in animal evolution. We collected in total over 100 vertebrate TA-receptor sequences, and our phylogenetic analysis shows that several TA-receptors have evolved rapidly with remarkable species variation and that the common ancestor of vertebrate TA-receptors arose before the split of the ray-finned and lobe-finned fishes. The evolutionary history of the TA-receptors is more complex than for most other GPCR families and here we suggest a mechanism by which they may have arisen.
AB - Trace amines, such as tyramine, beta-phenylethylamine, tryptamine, and octopamine, are present in trace levels in nervous systems and bind a specific family of G-protein-coupled receptors (GPCR), but the function or origin of this system is not well understood. We searched the genomes of several eukaryotic species for receptors similar to the mammalian trace amine (TA) receptor subfamily. We identified 18 new receptors in rodents that are orthologous to the previously known TA-receptors. Remarkably, we found 57 receptors (and 40 pseudogenes) of this type in the zebrafish (Danio rerio), while fugu (Takifugu rubripes) had only eight receptors (and seven pseudogenes). We mapped 47 of the zebrafish TA-receptors on chromosomes using radiation hybrid panels and meiotic mapping. The results, together with the degree of conservation and phylogenetic relationships displayed among the zebrafish receptors suggest that the family arose through several different mechanisms involving tetraploidization, block duplications, and local duplication events. Interestingly, these vertebrate TA-receptors do not show a close evolutionary relationship to the invertebrate TA-binding receptors in fruitfly (Drosophila melanogaster), indicating that the ability to bind TA have evolved at least twice in animal evolution. We collected in total over 100 vertebrate TA-receptor sequences, and our phylogenetic analysis shows that several TA-receptors have evolved rapidly with remarkable species variation and that the common ancestor of vertebrate TA-receptors arose before the split of the ray-finned and lobe-finned fishes. The evolutionary history of the TA-receptors is more complex than for most other GPCR families and here we suggest a mechanism by which they may have arisen.
KW - Amino Acid Sequence
KW - Animals
KW - Chromosome Mapping
KW - Conserved Sequence
KW - Expressed Sequence Tags
KW - Humans
KW - Likelihood Functions
KW - Markov Chains
KW - Mice
KW - Molecular Sequence Data
KW - Nervous System
KW - Octopamine
KW - Phenethylamines
KW - Phylogeny
KW - Rats
KW - Receptors, G-Protein-Coupled
KW - Sequence Alignment
KW - Sequence Homology, Amino Acid
KW - Tryptamines
KW - Tyramine
KW - Zebrafish
KW - Zebrafish Proteins
U2 - 10.1016/j.ympev.2004.12.003
DO - 10.1016/j.ympev.2004.12.003
M3 - Journal article
C2 - 15804416
VL - 35
SP - 470
EP - 482
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
SN - 1055-7903
IS - 2
ER -
ID: 45811676