Locked nucleic acid: a potent nucleic acid analog in therapeutics and biotechnology
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Locked nucleic acid : a potent nucleic acid analog in therapeutics and biotechnology. / Stenvang, Jan; Sørensen, Mads D; Wengel, Jesper.
In: Oligonucleotides, Vol. 14, No. 2, 2004, p. 130-46.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Locked nucleic acid
T2 - a potent nucleic acid analog in therapeutics and biotechnology
AU - Stenvang, Jan
AU - Sørensen, Mads D
AU - Wengel, Jesper
PY - 2004
Y1 - 2004
N2 - Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.
AB - Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.
KW - Animals
KW - Biotechnology
KW - Drug Design
KW - Humans
KW - Oligonucleotides
KW - Oligonucleotides, Antisense
KW - Ribonuclease H
U2 - 10.1089/1545457041526317
DO - 10.1089/1545457041526317
M3 - Journal article
C2 - 15294076
VL - 14
SP - 130
EP - 146
JO - Nucleic Acid Therapeutics
JF - Nucleic Acid Therapeutics
SN - 2159-3337
IS - 2
ER -
ID: 59324800