Bacteria produce many kinds of volatile compounds throughout their lifecycle. Identifying these volatile compounds can help to understand bacterial interactions with the host and/or other surrounding pathogens of the same or different species. Some commonly used techniques to detect these volatile compounds are GC and/or LC coupled to mass spectrometric techniques. However, these methods can sometimes become challenging owing to tedious sample preparation steps. Thus, identifying an easier method to detect these volatile compounds was investigated in the present study. Here, Membrane-inlet mass spectrometry (MIMS) provided a facile low-impact alternative to the existing strategies. MIMS was able to differentiate between the pathogenic and nonpathogenic bacterial strains, implying that it can be used as a bioprocess monitoring tool to analyze water samples from either water treatment plants or biotechnological industries.