Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis
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Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis. / Skorda, Aikaterini; Lauridsen, Anna Røssberg; Huhtinen, Kaisa; Lahtinen, Alexandra; Senkowski, Wojciech; Oikkonen, Jaana; Hynninen, Johanna; Hautaniemi, Sampsa; Kallunki, Tuula.
In: STAR Protocols, Vol. 4, No. 4, 102683, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis
AU - Skorda, Aikaterini
AU - Lauridsen, Anna Røssberg
AU - Huhtinen, Kaisa
AU - Lahtinen, Alexandra
AU - Senkowski, Wojciech
AU - Oikkonen, Jaana
AU - Hynninen, Johanna
AU - Hautaniemi, Sampsa
AU - Kallunki, Tuula
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2023
Y1 - 2023
N2 - Patient-derived organoids (PDOs) are ideal ex vivo model systems to study cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring drug efficacy in three-dimensional (3D) high-grade serous ovarian cancer PDO cultures through quantification of cytotoxicity using propidium iodide incorporation in dead cells. We also provide detailed steps to analyze proliferation of PDOs using the Ki67 biomarker. We describe steps for sample processing, immunofluorescent staining, high-throughput confocal imaging, and image-based quantification for 3D cultures. For complete details on the use and execution of this protocol, please refer to Lahtinen et al. (2023).1
AB - Patient-derived organoids (PDOs) are ideal ex vivo model systems to study cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring drug efficacy in three-dimensional (3D) high-grade serous ovarian cancer PDO cultures through quantification of cytotoxicity using propidium iodide incorporation in dead cells. We also provide detailed steps to analyze proliferation of PDOs using the Ki67 biomarker. We describe steps for sample processing, immunofluorescent staining, high-throughput confocal imaging, and image-based quantification for 3D cultures. For complete details on the use and execution of this protocol, please refer to Lahtinen et al. (2023).1
KW - Cancer
KW - Cell Biology
KW - High-Throughput Screening
KW - Organoids
U2 - 10.1016/j.xpro.2023.102683
DO - 10.1016/j.xpro.2023.102683
M3 - Journal article
C2 - 37976153
AN - SCOPUS:85176911535
VL - 4
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 4
M1 - 102683
ER -
ID: 374454942