Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain. / Verma, A; Hirsch, D J; Hanley, M R; Thastrup, Ole; Christensen, Søren Brøgger; Snyder, S H.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 1990, p. 811-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Verma, A, Hirsch, DJ, Hanley, MR, Thastrup, O, Christensen, SB & Snyder, SH 1990, 'Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain', Biochemical and Biophysical Research Communications, vol. 172, no. 2, pp. 811-6.

APA

Verma, A., Hirsch, D. J., Hanley, M. R., Thastrup, O., Christensen, S. B., & Snyder, S. H. (1990). Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain. Biochemical and Biophysical Research Communications, 172(2), 811-6.

Vancouver

Verma A, Hirsch DJ, Hanley MR, Thastrup O, Christensen SB, Snyder SH. Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain. Biochemical and Biophysical Research Communications. 1990;172(2):811-6.

Author

Verma, A ; Hirsch, D J ; Hanley, M R ; Thastrup, Ole ; Christensen, Søren Brøgger ; Snyder, S H. / Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 2. pp. 811-6.

Bibtex

@article{200e8e8b504d41048f674b02f8626638,
title = "Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain",
abstract = "ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.",
keywords = "Animals, Brain, Calcimycin, Calcium, Carcinogens, Endoplasmic Reticulum, Heparin, Inositol 1,4,5-Trisphosphate, Kinetics, Microsomes, Organ Specificity, Rats, Terpenes, Thapsigargin",
author = "A Verma and Hirsch, {D J} and Hanley, {M R} and Ole Thastrup and Christensen, {S{\o}ren Br{\o}gger} and Snyder, {S H}",
year = "1990",
language = "English",
volume = "172",
pages = "811--6",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

AU - Verma, A

AU - Hirsch, D J

AU - Hanley, M R

AU - Thastrup, Ole

AU - Christensen, Søren Brøgger

AU - Snyder, S H

PY - 1990

Y1 - 1990

N2 - ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

AB - ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

KW - Animals

KW - Brain

KW - Calcimycin

KW - Calcium

KW - Carcinogens

KW - Endoplasmic Reticulum

KW - Heparin

KW - Inositol 1,4,5-Trisphosphate

KW - Kinetics

KW - Microsomes

KW - Organ Specificity

KW - Rats

KW - Terpenes

KW - Thapsigargin

M3 - Journal article

C2 - 2241971

VL - 172

SP - 811

EP - 816

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 43349744