Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain
Research output: Contribution to journal › Journal article › Research › peer-review
ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.
Original language | English |
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Journal | Biochemical and Biophysical Research Communications |
Volume | 172 |
Issue number | 2 |
Pages (from-to) | 811-6 |
Number of pages | 6 |
ISSN | 0006-291X |
Publication status | Published - 1990 |
- Animals, Brain, Calcimycin, Calcium, Carcinogens, Endoplasmic Reticulum, Heparin, Inositol 1,4,5-Trisphosphate, Kinetics, Microsomes, Organ Specificity, Rats, Terpenes, Thapsigargin
Research areas
ID: 43349744