Ethnopharmacological relevance: The roots of Rubia tibetica are chewed as an antidote to anaphylaxis caused by bites of the tick Ornithodoros lahorensis by the Wakhi people in Afghanistan. Aims of the study: To test whether Rubia tibetica possess anti-histamine effect. Materials and methods: Water and ethanol extracts of roots of Rubia tibetica were tested for anti-histamine effect on the H ₁-receptor in the guinea pig ileum assay. Fixed concentrations of plant extract with increasing concentrations of histamine were examined. Mepyramine was used as control. Results and conclusion: The ethanol extract of Rubia tibetica showed dose-dependent anti-histamine effect, whereas the water extract had little activity. The chewing of Rubia tibetica roots may alleviate the fatal swelling of the tongue during anaphylaxis.